A novel method for simultaneous high resolution identification of HLA-A, HLA-B, and HLA-Cw alleles

被引:26
作者
Arguello, R
Avakian, H
Goldman, JM
Madrigal, JA
机构
[1] ROYAL FREE HOSP,ANTHONY NOLAN RES INST,LONDON NW3 2QG,ENGLAND
[2] ROYAL FREE HOSP,SCH MED,DEPT HEMATOL,LONDON NW3 2QG,ENGLAND
[3] ROYAL POSTGRAD MED SCH,DEPT HEMATOL,LONDON W12 0NN,ENGLAND
关键词
D O I
10.1073/pnas.93.20.10961
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We describe a novel high resolution DNA based typing approach for HLA class I alleles, which identifies the recombinational motifs present in exons 2 and 3 of the HLA class I genes. Unique identification patterns for 201 known HLA-A, HLA-B, and HLA-Cw alleles were generated by the use of only 40 probes, which were targeted at these common motifs. The unambiguous identification of the alleles was achieved by the development of a new and powerful allelic separation technique that allows isolation of single alleles after amplification. To validate the method, we have used locus-specific primers to amplify exons 2 and 3 of HLA-A, HLA-B, and HLA-Cw loci from 22 heterozygous and 41 homozygous cell lines. After amplification, the allelic fragments from each locus were separated, blotted, and hybridized with the 40 probes. In all cases, the allelic products could be separated and 81 different class I alleles, 33 HLA-A, 30 HLA-B, and 18 HLA-Cw, were identified according to the predicted probe hybridization patterns.
引用
收藏
页码:10961 / 10965
页数:5
相关论文
共 22 条
  • [11] GARAGHTY DE, 1992, J IMMUNOL, V149, P1934
  • [12] HILDEBRAND WH, 1992, J IMMUNOL, V148, P1155
  • [13] POLYMERASE CHAIN-REACTION SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS OF POLYMORPHISM IN DPA1 AND DPB1 GENES - A SIMPLE, ECONOMICAL, AND RAPID METHOD FOR HISTOCOMPATIBILITY TESTING
    HOSHINO, S
    KIMURA, A
    FUKUDA, Y
    DOHI, K
    SASAZUKI, T
    [J]. HUMAN IMMUNOLOGY, 1992, 33 (02) : 98 - 107
  • [14] DEFINING THE COMMON SUBTYPES OF HLA A9, A10, A28 AND A19 BY USE OF ARMS/PCR
    KRAUSA, P
    BODMER, JG
    BROWNING, MJ
    [J]. TISSUE ANTIGENS, 1993, 42 (02): : 91 - 99
  • [15] GENETIC-POLYMORPHISM WITHIN HLA-A-ASTERISK-02 - SIGNIFICANT ALLELIC VARIATION REVEALED IN DIFFERENT POPULATIONS
    KRAUSA, P
    BRYWKA, M
    SAVAGE, D
    HUI, KM
    BUNCE, M
    NGAI, JLF
    TEO, DLT
    ONG, YW
    BAROUCH, D
    ALLSOP, CEM
    HILL, AVS
    MCMICHAEL, AJ
    BODMER, JG
    BROWNING, MJ
    [J]. TISSUE ANTIGENS, 1995, 45 (04): : 223 - 231
  • [16] OCHS LA, 1994, BONE MARROW TRANSPL, V13, P455
  • [17] THE ORIGINS OF HLA-A,B,C POLYMORPHISM
    PARHAM, P
    ADAMS, EJ
    ARNETT, KL
    [J]. IMMUNOLOGICAL REVIEWS, 1995, 143 : 141 - 180
  • [18] A COMPREHENSIVE APPROACH FOR TYPING THE ALLELES OF THE HLA-B LOCUS BY AUTOMATED SEQUENCING
    PETERSDORF, EW
    HANSEN, JA
    [J]. TISSUE ANTIGENS, 1995, 46 (02): : 73 - 85
  • [19] SMYTH LA, 1993, BONE MARROW TRANSPL, V12, P351
  • [20] SUBGROUPING OF DR4 ALLELES BY DNA HETERODUPLEX ANALYSIS
    SORRENTINO, R
    CASCINO, I
    TOSI, R
    [J]. HUMAN IMMUNOLOGY, 1992, 33 (01) : 18 - 23