Validated method for the simultaneous determination of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-THC and 11-nor-9-carboxy-THC in human plasma using solid phase extraction and gas chromatography-mass spectrometry with positive chemical ionization

被引:74
作者
Gustafson, RA
Moolchan, ET
Barnes, A
Levine, B
Huestis, MA
机构
[1] NIDA, Intramural Res Program, NIH, Baltimore, MD 21224 USA
[2] Univ Maryland, Dept Epidemiol & Prevent Med, Baltimore, MD 21201 USA
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 798卷 / 01期
关键词
Delta(9)-tetrahydrocannabinol; 11-hydroxy-Delta(9)-tetrahydrocannabinol; 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol;
D O I
10.1016/j.jchromb.2003.09.022
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A fully validated, highly sensitive and specific method for the extraction and quantification of Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-Delta(9)-tetrahydrocannabinol (11-OH-THC) and 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinoI (THCCOOH) in plasma is presented. This method incorporates Escherichia coli beta-glucuronidase hydrolysis to cleave glucuronic acid moieties to capture total analyte concentrations, and simultaneous solid phase extraction (SPE) of the three analytes in a single eluant with separation and quantification on a bench-top positive chemical ionization (PCI) gas chromatography-mass spectrometry (GC-MS) in the selected ion monitoring (SIM) mode. Quantitation was achieved by the addition of deuterated analogues for each analyte as internal standards (IS). Limits of quantitation (LOQ) were 0.5, 0.5 and 1.0 for THC, 11-OH-THC and THCCOOH, respectively, with linearity ranging up to 50 ng/ml for THC and 11-OH-THC, and 100 ng/ml for THCCOOH. Absolute recoveries ranged from 67.3 to 83.5% for all three analytes. Intra-assay accuracy and precision ranged from 1.2 to 12.2 and 1.4 to 4.7%, respectively. Inter-assay accuracy and precision ranged from 1.4 to 12.2 and 3.1 to 7.3%, respectively. This method was used to analyze plasma samples collected from individuals participating in a controlled oral THC administration study. Statistically significant (P less than or equal to 0.05) increases of 40% for 11-OH-THC and 42% for THCCOOH concentrations were found between hydrolyzed and non-hydrolyzed results. This method will be utilized in ongoing controlled cannabinoid administration studies and may be a useful analytical procedure for the fields of forensic toxicology and cannabinoid pharmacology. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:145 / 154
页数:10
相关论文
共 33 条
[1]  
AGURELL S, 1986, PHARMACOL REV, V38, P21
[2]   Positive cannabis results in urine and blood samples after consumption of hemp food products [J].
Alt, A ;
Reinhardt, G .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1998, 22 (01) :80-81
[3]   An automated and simultaneous solid-phase extraction of Δ9-tetrahydrocannabinol and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol from whole blood using the Zmark RapidTrace™ with confirmation and quantitation by GC-El-MS [J].
D'Asaro, JA .
JOURNAL OF ANALYTICAL TOXICOLOGY, 2000, 24 (04) :289-295
[4]   MEDICAL USE OF MARIJUANA [J].
DOBLIN, R ;
KLEIMAN, MAR .
ANNALS OF INTERNAL MEDICINE, 1991, 114 (09) :809-810
[5]   Δ9-THC metabolites in meconium:: Identification of 11-OH-Δ9-THC, 8β,11-diOH-Δ9-THC, and 11-nor-Δ9-THC-9-COOH as major metabolites of Δ9-THC [J].
ElSohly, MA ;
Feng, SX .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1998, 22 (04) :329-335
[6]   Simultaneous analysis of Δ9-THC and its major metabolites in urine, plasma, and meconium by GC-MS using an immunoaffinity extraction procedure [J].
Feng, SX ;
ElSohly, MA ;
Salamone, S ;
Salem, MY .
JOURNAL OF ANALYTICAL TOXICOLOGY, 2000, 24 (06) :395-402
[7]  
FOLTZ RL, 1984, ADV ANAL TOXICOLOGY, P125
[8]   Marijuana-positive urine test results from consumption of hemp seeds in food products [J].
Fortner, N ;
Fogerson, R ;
Lindman, D ;
Iversen, T ;
Armbruster, D .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1997, 21 (06) :476-481
[9]  
GUSTAFSON RA, 2003, CLIN CHEM
[10]  
HARVEY DJ, 1984, REV BIOCHEM TOXICOL, V6, P221