Application of nine-color flow cytometry for detailed studies of the phenotypic complexity and functional heterogeneity of human lymphocyte subsets

被引：22

作者：

Gonzalez, Veronica D. ^{[1
]}

Bjorkstroem, Niklas K. ^{[1
]}

Malmberg, Karl-Johan ^{[1
]}

Moll, Markus ^{[1
]}

Kuylenstierna, Carlotta ^{[1
]}

Michalsson, Jakob ^{[1
]}

Ljunggren, Hans-Gustaf ^{[1
]}

Sandberg, Johan K. ^{[1
]}

机构：

[1] Karolinska Univ Hosp Huddinge, Karolinska Inst, Ctr Infect Dis, Dept Med,CIM, S-14186 Stockholm, Sweden

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2008年 / 330卷 / 1-2期

关键词：

flow cytometry; FACS; T cells; NKT cells; NK cells; CD1d;

D O I：

10.1016/j.jim.2007.10.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Innate and adaptive cellular immunity is initiated, directed and regulated by a vast array of cell surface receptors. Attempts to harness the cellular immune system in translational settings such as immunotherapy and vaccine development require tools to accurately describe and isolate lymphocytes with specific characteristics. One such tool, flow cytometry, is undergoing a revolution in instrumentation and reagents, providing opportunities for high resolution phenotypic and functional analysis of lymphocytes. Here, we demonstrate how nine-color flow cytometry can be adapted, optimized and applied to investigate the phenotypic complexity and functional heterogeneity of human lymphocyte subsets. We provide examples of studies of adaptive T cell responses against viruses, as well as the assessment of CD1d-restricted NKT cells and NK cells. We discuss the importance of this technology for detailed investigations of lymphocyte subsets in studies of infectious diseases and cancer. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：64 / 74

页数：11

共 37 条

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