Profiling of N- and O-glycopeptides of erythropoietin by capillary zwitterionic type of hydrophilic interaction chromatography/electrospray ionization mass spectrometry

被引：58

作者：

Takegawa, Yasuhiro ^{[1
]}

Ito, Hiroki ^{[1
,2
]}

Keira, Takuro ^{[1
]}

Deguchi, Kisaburo ^{[1
]}

Nakagawa, Hiroaki ^{[1
]}

Nishimura, Shin-Ichiro ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Adv Life Sci, Sapporo, Hokkaido 0010021, Japan

[2] Hitachi High Technol Co, Hitachinaka, Ibaraki, Japan

来源：

JOURNAL OF SEPARATION SCIENCE | 2008年 / 31卷 / 09期

关键词：

erythropoietin; glycopeptides; hydrophilic interaction chromatography; mass spectrometry; zwitterionic interaction column;

D O I：

10.1002/jssc.200700679

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary zwitterionic-type hydrophilic interaction chromatography (ZIC-HILIC)/ESI-MS has been applied to the Glu-C digest of recombinant human erythropoietin (rhEPO) expressed in Chinese hamster ovary (CHO) cells. N-Glycopeptides (105) and O-glycopeptides (8) were detected in a single run of the capillary ZIC-HILIC/ESI-MS analysis. Among them, N-acetyl-neuraminic acids (Neu5Ac) of N- and O-glycans were partially acetylated and some were replaced with N-glycoyl-neuraminic acid (Neu5Gc). Their retentions in the ZIC-HILIC separation can be explained to some extent with the degree of acetylation and N-glycoylation, both of which influence the hydrophilicity/hydrophobicity of the N- and O-glycan moieties of glycopeptides.

引用

页码：1585 / 1593

页数：9

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