Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library

被引:61
作者
Hu, HB
Li, L
Kao, RY
Kou, BB
Wang, ZG
Zhang, L
Zhang, HY
Hao, ZY
Tsui, WH
Ni, AP
Cui, LX
Fan, BX
Guo, F
Rao, S
Jiang, CY
Li, Q
Sun, MJ
He, W
Liu, G
机构
[1] Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China
[2] Peking Union Med Coll, Beijing 100050, Peoples R China
[3] Chinese Acad Med Sci, Dept Immunol, Inst Basic Med Sci, Beijing 100005, Peoples R China
[4] Peking Union Med Coll, Sch Basic Med Sci, Beijing 100005, Peoples R China
[5] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
[6] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China
[7] Peking Union Med Coll, Beijing 100730, Peoples R China
[8] Gen Hosp PLA, Sci & Technol Convers Ctr, Beijing 100853, Peoples R China
[9] Acad Mil Med Sci, Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
来源
JOURNAL OF COMBINATORIAL CHEMISTRY | 2005年 / 7卷 / 05期
关键词
D O I
10.1021/cc0500607
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A 10-mer overlapping peptide library has been synthesized for screening and identification of linear B-cell epitopes of severe acute respiratory syndrome associated coronavirus (SARS-CoV), which spanned the major structural proteins of SARS-CoV. One hundred and eleven candidate peptides were positive according to the result of PEPscan, which were assembled into 22 longer peptides. Five of these peptides showed high cross-immunoreactivities (similar to 66.7 to 90.5%) to SARS convalescent patients' sera from the severest epidemic regions of the China mainland. Most interestingly, S471-503, a peptide located at the receptor binding domain (RBD) of SARS-CoV, could specifically block the binding between the RBD and angiotensin-converting enzyme 2, resulting in the inhibition of SARS-CoV entrance into host cells in vitro. The study demonstrated that S471-503 peptide was a potential immunoantigen for the development of peptide-based vaccine or a candidate for further drug, evaluation against the SARS-CoV virus-cell fusion.
引用
收藏
页码:648 / 656
页数:9
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