Supplementation with vitamin C and N-acetyl-cysteine increases oxidative stress in humans after an acute muscle injury induced by eccentric exercise

被引:268
作者
Childs, A
Jacobs, C
Kaminski, T
Halliwell, B
Leeuwenburgh, C
机构
[1] Univ Florida, Coll Hlth & Human Performance, Ctr Exercise Sci, Biochem Aging Lab, Gainesville, FL 32611 USA
[2] Natl Univ Singapore, Dept Biochem, Singapore 117548, Singapore
关键词
pro-oxidants; ascorbic acid; neutrophils; sepsis; free radicals; disease; antioxidants;
D O I
10.1016/S0891-5849(01)00640-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There has been no investigation to determine if the widely used over-the-counter, water-soluble antioxidants vitamin C and N-acetyl-cysteine (NAC) could act as pro-oxidants in humans during inflammatory conditions. We induced an acute-phase inflammatory response by an eccentric arm muscle injury. The inflammation was characterized by edema, swelling, pain, and increases in plasma inflammatory indicators, myeloperoxidase and interleukin-6. Immediately following the injury, subjects consumed a placebo or vitamin C (12.5 mg/kg body weight) and NAC (10 mg/kg body weight) for 7 d. The resulting muscle injury caused increased levels of serum bleomycin-detectable iron and the amount of iron was higher in the vitamin C and NAC group. The concentrations of lactate dehydrogenase (LDH), creatine kinase. (CK), and myoglobin were significantly elevated 2, 3, and 4 d postinjury and returned to baseline levels by day 7. In addition, LDH and CK activities were elevated to a greater extent in the vitamin C and NAC group. Levels of markers for oxidative stress (lipid hydroperoxides and 8-iso prostaglandin F-2 alpha; 8-Iso-PGF(2 alpha)) and antioxidant enzyme activities were also elevated post-injury. The subjects receiving vitamin C and NAC had higher levels of lipid hydroperoxides and 8-Iso-PGF(2 alpha) 2 d after the exercise. This acute human inflammatory model strongly suggests that vitamin C and NAC supplementation immediately post-injury, transiently increases tissue damage and oxidative stress. (C) 2001 Elsevier Science Inc.
引用
收藏
页码:745 / 753
页数:9
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