Phorbol esters promote postsynaptic accumulation of Vesl-1S/Homer-1a protein

被引:29
作者
Kato, A [1 ]
Fukuda, T
Fukazawa, Y
Isojima, Y
Fujitani, K
Inokuchi, K
Sugiyama, H
机构
[1] Kyushu Univ, Grad Sch Sci, Dept Biol, Higashi Ku, Fukuoka 8128581, Japan
[2] Kyushu Univ, Grad Sch Med Sci, Dept Anat & Neurobiol, Higashi Ku, Fukuoka 8128582, Japan
[3] Mitsubishi Kasei Inst Life Sci, Machida, Tokyo 1948511, Japan
[4] Osaka Univ, Inst Prot Res, Div Prot Metab, Suita, Osaka 5650871, Japan
关键词
immunocytochemistry; rat; subcellular distribution; vesl-1;
D O I
10.1046/j.0953-816x.2001.01498.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We examined effects of phorbol esters on the amount and the subcellular distribution of the activity-regulated protein Vesl-1S/ Homer-la in cultured hippocampal neurons. Major Vesl-1S immunoreactivity (IR) was detected throughout neuronal somata under control conditions. Bath application of phorbol esters, PMA and PDBU resulted in the increase in the amount of Vesl-1S proteins and promoted punctate distribution of Vesl-1S IR at the cortical regions of the neuronal somata. Immunofluorescent observations using antisynaptophysin and anti-Vesl-1S antibodies, and electron microscopic observations, revealed that Vesl-1S accumulated at postsynaptic regions following PMA application. Membrane depolarization with high concentrations of external potassium also promoted the punctate distribution of Vesl-1S IR. These results demonstrate that phorbol-triggered reaction cascades result in the accumulation of Ves-1S protein at postsynaptic regions, and suggest that these phorbol effects may mimic those caused by synaptic activities.
引用
收藏
页码:1292 / 1302
页数:11
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