Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases

被引:386
作者
Heid, HW
Moll, R
Schwetlick, I
Rackwitz, HR
Keenan, TW
机构
[1] German Canc Res Ctr, Div Cell Biol, D-69120 Heidelberg, Germany
[2] Univ Halle Wittenberg, Inst Pathol, D-06097 Halle, Germany
[3] Virginia Polytech Inst & State Univ, Dept Biochem, Blacksburg, VA 24061 USA
关键词
adipophilin; lipid droplets; fat-accumulating cells; lipid-binding protein;
D O I
10.1007/s004410051181
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We report the human DNA and protein sequence of adipophilin and its association with the surface of lipid droplets. The amino acid sequence of human adipophilin has been determined by using cDNA clones from several tissues and confirmed by the reverse transcription/polymerase chain reaction method and Edman sequencing. The open reading frame of adipophilin encodes a polypeptide with a calculated molecular weight of 48.1 kDa and an isoelectric point of 6.72. By immunofluorescence and electron-microscopic localization with newly raised specific poly- and monoclonal antibodies, we show that this protein is not restricted to adipocytes as previously indicated by studies of the mouse homologous protein, adipose-differentiation-related protein. Adipophilin occurs in a wide range of cultured cell lines, including fibroblasts and endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to certain cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. Our results reveal adipophilin as a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
引用
收藏
页码:309 / 321
页数:13
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