Continuous flow displacement immunosensors: A computational study

被引：13

作者：

Holt, DB ^{[1
]}

Kusterbeck, AW ^{[1
]}

Ligler, FS ^{[1
]}

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2000年 / 287卷 / 02期

关键词：

computational modeling; biosensor; displacement immunoassay;

D O I：

10.1006/abio.2000.4856

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Numerical modeling has been used to investigate the disparity in performance and sensitivity that has been reported for flow displacement immunosensors based on bead-packed columns, membranes, and capillary tubes. The simulations strongly suggest that the high surface areas in the porous media systems may actually be detrimental to sensor performance because of large numbers of free antibody binding sites. Since the free antibody sites are created during the wash step in which the baseline is established, wash protocols are critical in optimizing the sensitivity for a given displacement sensor.

引用

页码：234 / 242

页数：9

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