Chromatographic purification and characterization of B-phycoerythrin from Porphyridium cruentum -: Semipreparative high-performance liquid chromatographic separation and characterization of its subunits

被引：52

作者：

Bermejo, R

Talavera, EM

Alvarez-Pez, JM ^{[1
]}

机构：

[1] Univ Granada, Dept Chem Phys, E-18071 Granada, Spain

[2] Univ Jaen, Dept Phys & Analyt Chem, EUP Linares, Linares 23700, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 917卷 / 1-2期

关键词：

Porphyridium cruentum; phycoerythrins; biliproteins; proteins;

D O I：

10.1016/S0021-9673(01)00692-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A fast preparative two-step chromatographic method for purification of B-phycoerythrin from Porphyridium cruentum is described. This biliprotein was homogeneous as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielding three closely migrating bands corresponding to its three subunits. Baseline separation of its alpha-, beta- and gamma -subunits was achieved by a reversed-phase HPLC gradient semipreparative method with a C, large-pore column and a solvent system consisting of 0.05% trifluoroacetic acid (TFA) in water and 0.05% TFA in acetonitrile. B-Phycoerythrin in different aggregation states and its subunits have been spectroscopically characterized. Hexameric B-phycoerythrin has similar secondary and tertiary structure than dissociated B-phycoerythrin determined by circular dichroism. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：135 / 145

页数：11

共 34 条

[1]

Arad S., 1992, Trends in Food Science & Technology, V3, P92, DOI 10.1016/0924-2244(92)90145-M

[2]

MOLECULAR ASSEMBLY OF PROTEINS AND CONJUGATED POLYMERS - TOWARD DEVELOPMENT OF BIOSENSORS [J].