Transgenic tobacco cultivars resistant to Pseudomonas syringae pv. tabaci

被引：17

作者：

Batchvarova, R ^{[1
]}

Nikolaeva, V

Slavov, S

Bossolova, S

Valkov, V

Atanassova, S

Guelemerov, S

Atanassov, A

Anzai, H

机构：

[1] De Montfort Univ, Norman Borlaug Ctr Plant Sci, Inst Genet Engn, Kostinbrod 2232 2, Bulgaria

[2] Bulgartabac Holding Grp, Sofia, Bulgaria

[3] Meiji Seika Kaisha Ltd, Pharmaceut Res Ctr, Yokohama, Kanagawa 222, Japan

来源：

THEORETICAL AND APPLIED GENETICS | 1998年 / 97卷 / 5-6期

关键词：

tobacco; Pseudomonas syringae pv. tabuci; transformation; resistance; inheritance;

D O I：

10.1007/s001220050981

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Six oriental cultivars of tobacco (Nicotiana tabacum L.) were evaluated for transformation and foreign gene expression. Leaf-disc explant tissue was transformed with Agrobacterium tumefaciens strain LBA4404 carrying the plasmid pARK21, which contains NPTII gene and ttr (tabtoxin resistance) gene conferring the resistance to Pseudomonas syringe pv, tabaci. The disease resistance of regenerated plants and segregation of this trait up to R-7 progeny were investigated in a greenhouse and under field conditions. Our results indicated that the resistance to Pseudomonas syringae pv. tabaci introduced by transformation is heritable.

引用

页码：986 / 989

页数：4

共 20 条

[1] TRANSGENIC TOBACCO RESISTANT TO A BACTERIAL DISEASE BY THE DETOXIFICATION OF A PATHOGENIC TOXIN [J].

ANZAI, H ;

YONEYAMA, K ;

YAMAGUCHI, I .

MOLECULAR & GENERAL GENETICS, 1989, 219 (03) :492-494

[2]

Daub ME, 1994, TOBACCO SCI, V38, P51

[3] ENGINEERING HERBICIDE RESISTANCE IN PLANTS BY EXPRESSION OF A DETOXIFYING ENZYME [J].

DEBLOCK, M ;

BOTTERMAN, J ;

VANDEWIELE, M ;

DOCKX, J ;

THOEN, C ;

GOSSELE, V ;

MOVVA, NR ;

THOMPSON, C ;

VANMONTAGU, M ;

LEEMANS, J .

EMBO JOURNAL, 1987, 6 (09) :2513-2518

[4]

DEGREEF W, 1989, BIO-TECHNOL, V7, P61, DOI 10.1038/nbt0189-61

[5] CHARACTERIZATION OF RNA-MEDIATED RESISTANCE TO TOMATO SPOTTED WILT VIRUS IN TRANSGENIC TOBACCO PLANTS [J].

DEHAAN, P ;

GIELEN, JJL ;

PRINS, M ;

WIJKAMP, IG ;

VANSCHEPEN, A ;

PETERS, D ;

VANGRINSVEN, MQJM ;

GOLDBACH, R .

BIO-TECHNOLOGY, 1992, 10 (10) :1133-1137

[6]

DELLAPORTA SL, 1983, PLANT MOL BIOL REP, V4, P419

[7] EXPRESSION OF A POTYVIRUS NON-STRUCTURAL PROTEIN IN TRANSGENIC TOBACCO [J].

GRAYBOSCH, R ;

HELLMANN, GM ;

SHAW, JG ;

RHOADS, RE ;

HUNT, AG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 160 (02) :425-432

[8] A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS [J].

HORSCH, RB ;

FRY, JE ;

HOFFMANN, NL ;

EICHHOLTZ, D ;

ROGERS, SG ;

FRALEY, RT .

SCIENCE, 1985, 227 (4691) :1229-1231

[9] PATHOGEN-DERIVED RESISTANCE TO A POTYVIRUS - IMMUNE AND RESISTANT PHENOTYPES IN TRANSGENIC TOBACCO EXPRESSING ALTERED FORMS OF A POTYVIRUS COAT PROTEIN NUCLEOTIDE-SEQUENCE [J].

LINDBO, JA ;

DOUGHERTY, WG .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1992, 5 (02) :144-153

[10] EXPRESSION OF ALFALFA MOSAIC-VIRUS RNA-4 IN TRANSGENIC PLANTS CONFERS VIRUS-RESISTANCE [J].

LOESCHFRIES, LS ;

MERLO, D ;

ZINNEN, T ;

BURHOP, L ;

HILL, K ;

KRAHN, K ;

JARVIS, N ;

NELSON, S ;

HALK, E .

EMBO JOURNAL, 1987, 6 (07) :1845-1851

← 1 2 →