PR-Set7-dependent methylation of histone H4 Lys 20 functions in repression of gene expression and is essential for mitosis

被引：146

作者：

Karachentsev, D

Sarma, K

Reinberg, D

Steward, R

机构：

[1] Rutgers State Univ, Waksman Inst, New Jersey Canc Ctr, Dept Mol Biol & Biochem, Piscataway, NJ 08854 USA

[2] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Howard Hughes Med Inst, Dept Biochem,Div Nucl Acid Enzymol, Piscataway, NJ 08854 USA

来源：

GENES & DEVELOPMENT | 2005年 / 19卷 / 04期

关键词：

histone methylation; facultative and constitutive heterochromatin; transcriptional repression; cell cycle and mitosis; Drosophila;

D O I：

10.1101/gad.1263005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The histone methyl transferase PR-Set7 mediates histone H4 Lys 20 methylation, a mark of constitutive and facultative heterochromatin. We isolated a null mutation in Drosophila PR-Set7 that suppresses position effect variegation, indicating that PR-Set7 indeed functions in silencing general gene expression. In PR-Set7 larval leg and eye discs, the number of cells is lower than normal, and the DNA content in these cells is significantly increased. These data show that PR-Set7-dependent methylation is essential for the process of mitosis. The methylation mark is highly stable and is maintained even in the absence of PR-Set7 protein.

引用

页码：431 / 435

页数：5

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