A DNA vaccine encoding Cu,Zn superoxide dismutase of Brucella abortus induces protective immunity in BALB/c mice

被引：89

作者：

Oñate, AA

Céspedes, S

Cabrera, A

Rivers, R

Gonzälez, A

Muñoz, C

Folch, H

Andrews, E

机构：

[1] Univ Concepcion, Fac Biol Sci, Dept Microbiol, Mol Immunol Lab, Concepcion, Chile

[2] Univ Austral Chile, Fac Med, Dept Immunol, Valdivia, Chile

来源：

INFECTION AND IMMUNITY | 2003年 / 71卷 / 09期

关键词：

D O I：

10.1128/IAI.71.9.4857-4861.2003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

This study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella abortus Cu,Zn superoxide dismutase (SOD). Intramuscular injection of plasmid DNA carrying the SOD gene (pcDNA-SOD) into BALB/c mice elicited both humoral and cellular immune responses. Animals injected with pcDNA-SOD developed SOD-specific antibodies which exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the DNA vaccine elicited a T-cell-proliferative response and also induced the production of gamma interferon, but not interleukin-10 (IL-10) or IL-4, upon restimulation with either recombinant SOD or crude Brucella protein, suggesting the induction of a typical T-helper-1-dominated immune response in mice. The pcDNA-SOD (but not the control vector) induced a strong, significant level of protection in BALB/c mice against challenge with B. abortus virulent strain 2308; the level of protection was similar to the one induced by B. abortus vaccine strain RB51. Altogether, these data suggest that pcDNA-SOD is a good candidate for use in future studies of vaccination against brucellosis.

引用

页码：4857 / 4861

页数：5

共 33 条

[1] Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp. [J].

Al-Mariri, A ;

Tibor, A ;

Mertens, P ;

De Bolle, X ;

Michel, P ;

Godfroid, JK ;

Walravens, K ;

Letesson, J .

INFECTION AND IMMUNITY, 2001, 69 (10) :6264-6270

[2]

BAE J, 1999, THESIS VIRGINIA POLY

[3]

BONA CA, 2000, GENETIC IMMUNIZATION, P11

[4] Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination [J].

Boyer, JD ;

Ugen, KE ;

Wang, B ;

Agadjanyan, M ;

Gilbert, L ;

Bagarazzi, ML ;

Chattergoon, M ;

Frost, P ;

Javadian, A ;

Williams, WV ;

Refaeli, Y ;

Ciccarelli, RB ;

McCallus, D ;

Coney, L ;

Weiner, DB .

NATURE MEDICINE, 1997, 3 (05) :526-532

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6] CLONING, EXPRESSION, AND OCCURRENCE OF THE BRUCELLA CU-ZN SUPEROXIDE-DISMUTASE [J].

BRICKER, BJ ;

TABATABAI, LB ;

JUDGE, BA ;

DEYOE, BL ;

MAYFIELD, JE .

INFECTION AND IMMUNITY, 1990, 58 (09) :2935-2939

[7] Brucellosis: An overview [J].

Corbel, MJ .

EMERGING INFECTIOUS DISEASES, 1997, 3 (02) :213-221

[8] DNA vaccines [J].

Donnelly, JJ ;

Ulmer, JB ;

Shiver, JW ;

Liu, MA .

ANNUAL REVIEW OF IMMUNOLOGY, 1997, 15 :617-648

[9]

Feltquate DM, 1997, J IMMUNOL, V158, P2278

[10] Immunity and protection against Brucella abortus [J].

Golding, B ;

Scott, DE ;

Scharf, O ;

Huang, LY ;

Zaitseva, M ;

Lapham, C ;

Eller, N ;

Golding, H .

MICROBES AND INFECTION, 2001, 3 (01) :43-48

← 1 2 3 4 →