Heteronuclear 2D-correlations in a uniformly [13C, 15N] labeled membrane-protein complex at ultra-high magnetic fields

被引:81
作者
Egorova-Zachernyuk, TA
Hollander, J
Fraser, N
Gast, P
Hoff, AJ
Cogdell, R
de Groot, HJM [1 ]
Baldus, M
机构
[1] Leiden Univ, Gorlaeus Labs, NL-2333 CC Leiden, Netherlands
[2] Leiden Univ, Huygens Lab, NL-2333 CC Leiden, Netherlands
[3] Univ Glasgow, Div Biochem & Mol Biol, Glasgow G12 8QQ, Lanark, Scotland
关键词
correlation spectroscopy; LH2 light-harvesting complex; MAS; membrane-proteins; photosynthetic bacteria; polarization transfer; spin-diffusion; solid-state NMR;
D O I
10.1023/A:1011235417465
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
One- and two-dimensional solid-state NMR experiments on a uniformly labeled intrinsic membrane-protein complex at ultra-high magnetic fields are presented. Two-dimensional backbone and side-chain correlations for a [U-C-13,N-15] labeled version of the LH2 light-harvesting complex indicate significant resolution at low temperatures and under Magic Angle Spinning. Tentative assignments of some of the observed correlations are presented and attributed to the alpha -helical segments of the protein, mostly found in the membrane interior.
引用
收藏
页码:243 / 253
页数:11
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