Wavelength-scanning surface plasmon resonance imaging

被引:30
作者
Otsuki, S
Tamada, K
Wakida, S
机构
[1] Natl Inst Adv Ind Sci & Technol, Single Mol Bioanal Lab, Takamatsu, Kagawa 7610395, Japan
[2] AIST, Photon Res Inst, Tsukuba, Ibaraki 3058565, Japan
[3] AIST, Human Stress Signal Res Ctr, Ikeda, Osaka 5638577, Japan
关键词
D O I
10.1364/AO.44.003468
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
A new surface plasmon resonance (SPR) imaging technique was proposed. After measurements were conducted at varying wavelengths, the wavelength affording the minimum brightness (SPR wavelength) was determined at each pixel of the image. A two-dimensional map of the SPR wavelength could be converted to a thickness profile by use of a nonlinear calibration curve, which was obtained by Fresnel calculation. An array of protein thin layers on a gold film was evaluated in air to present the layers' surface structure in nanometer scale. (c) 2005 Optical Society of America.
引用
收藏
页码:3468 / 3472
页数:5
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