Rectangular capillary electrophoresis: Study of some dispersive effects

被引：14

作者：

Cifuentes, A

Rodriguez, MA

GarciaMontelongo, FJ

机构：

[1] Depto. Quim. Analitica, B., Facultad de Químicas, Universidad de la Lagunna, 38204 La Laguna (Tenerife), Avenida Trinidad s/n

[2] Inst. de Quim. Orgánica, 28006 Madrid

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 737卷 / 02期

关键词：

heat generation; adsorption; siphoning effect; rectangular capillaries; dispersive effects;

D O I：

10.1016/0021-9673(96)00002-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Different phenomena occurring during capillary electrophoretic (CE) separations (i.e., heat generation, spontaneous injection, siphoning effect and adsorption of the solute on the capillary wall) were investigated in cylindrical and rectangular capillaries of large cross-sectional area. The equation that relates the plate height to the extent of adsorption in a rectangular duct is shown and compared with that for cylindrical capillaries. Theoretical and experimental results obtained with capillaries of both geometries are compared. The advantages and drawbacks of using rectangular capillaries in micropreparative CE are discussed. A simple modification to the commercial CE instrument used in this work allowed the easy installation of rectangular capillaries within the apparatus.

引用

页码：243 / 253

页数：11

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