Modification of an automated liquid-handling system for reagent-jet, nanoliter-level dispensing

被引：11

作者：

Hicks, JS ^{[1
]}

Harker, BW ^{[1
]}

Beattie, KL ^{[1
]}

Doktycz, MJ ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Div Life Sci, Oak Ridge, TN 37831 USA

来源：

BIOTECHNIQUES | 2001年 / 30卷 / 04期

关键词：

D O I：

10.2144/01304rr05

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reducing the scale of biochemical reactions is becoming commonplace. Examples include the screening of large libraries of chemical compounds or gene sequences. These applications demand the ability to transfer sub-microliter volumes of fluid. To this end, we have modified a Hamilton(R) MICROLAB(R) 2200 with high-speed solenoids and a liquid pressurization system to modulate volume delivery down to the nanoliter level. Additional modifications include the use of sapphire-tipped dispensing nozzles and a high-resolution substage to assist in the construction of DNA microarrays. Techniques for characterizing the dispensed volume are presented.

引用

页码：878 / +

页数：7

共 17 条

[1]

Bateman TA, 1999, LAB ROBOTICS AUTOMAT, V11, P250, DOI 10.1002/(SICI)1098-2728(1999)11:5<250::AID-LRA2>3.0.CO

[2]

2-1

[3]

BEATTIE KL, 1995, CLIN CHEM, V41, P700

[4] High-density oligonucleotide arrays [J].

Blanchard, AP ;

Kaiser, RJ ;

Hood, LE .

BIOSENSORS & BIOELECTRONICS, 1996, 11 (6-7) :687-690

[5] Multiplexed nanoliter transfers for high throughput drug screening using the Biomek 2000 and the High Density Replicating Tool [J].

Brandt, DW .

JOURNAL OF BIOMOLECULAR SCREENING, 1997, 2 (02) :111-116

[6] Making and reading microarrays [J].

Cheung, VG ;

Morley, M ;

Aguilar, F ;

Massimi, A ;

Kucherlapati, R ;

Childs, G .

NATURE GENETICS, 1999, 21 (Suppl 1) :15-19

[7] System for preparing microhybridization arrays on glass slides [J].

Graves, DJ ;

Su, HJ ;

McKenzie, SE ;

Surrey, S ;

Fortina, P .

ANALYTICAL CHEMISTRY, 1998, 70 (23) :5085-5092

[8]

KNEBEL G, 1998, AM BIOTECHNOL LAB, V3, P22

[9]

Le HP, 1998, J IMAGING SCI TECHN, V42, P49

[10] Characterization of an inkjet chemical microdispenser for combinatorial library synthesis [J].

Lemmo, AV ;

Fisher, JT ;

Geysen, HM ;

Rose, DJ .

ANALYTICAL CHEMISTRY, 1997, 69 (04) :543-551

← 1 2 →