A general ribonuclease assay using methylene blue

被引：46

作者：

GreinerStoeffele, T ^{[1
]}

Grunow, M ^{[1
]}

Hahn, U ^{[1
]}

机构：

[1] UNIV LEIPZIG,FAK BIOWISSENSCHAFT PHARM & PSYCHOL,INST BIOCHEM,D-04103 LEIPZIG,GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1996年 / 240卷 / 01期

关键词：

D O I：

10.1006/abio.1996.0326

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribonuclease (RNase) activity has been assayed by monitoring the shift in the absorbance maximum of methylene blue upon intercalation into high-molecular-weight RNA. After preincubation of yeast RNA with methylene blue, the initial rate of hydrolysis can be followed spectrophotometrically at a wavelength of 688 nm. This allows enzyme kinetic studies of RNases with their probable native substrate, RNA, in place of the artificial ones, such as dinucleoside phosphates or cyclic monophosphates, currently used. (C) 1996 Academic Press, Inc.

引用

页码：24 / 28

页数：5

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