A rapid, sensitive method for testing the activity of Escherichia coli O157:H7 secreted cytotoxins against epithelial cells from the jejunum and descending colon of cattle

被引：4

作者：

Baines, Danica ^{[1
]}

Masson, Luke ^{[2
]}

McAllister, Tim ^{[1
]}

机构：

[1] Agr & Agri Food Canada, Lethbridge Res Ctr, Lethbridge, AB T1J 4B1, Canada

[2] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada

来源：

CANADIAN JOURNAL OF ANIMAL SCIENCE | 2008年 / 88卷 / 01期

关键词：

Escherichia coli O157 : H7; cattle; intestine; cytotoxins; cytotoxicity;

D O I：

10.4141/CJAS07040

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Current methods for assessing Escherichia coli O157:H7 secreted cytotoxin activity are based on exposing human cell lines for 12 to 72 h and monitoring changes in cell morphology, adherence or enzyme release. These methods, although sensitive, use non-ruminant cell lines that may not represent cattle responses to E. coli O157:H7 secreted cytotoxins. The modified lawn assay used in this study was found to be a simple, fast and sensitive method for assessing cattle epithelial cell susceptibility to E. coli O157:H7 secreted cytotoxins within 4 to 6 h.

引用

页码：51 / 55

页数：5

共 20 条

[1]

Barkocy-Gallagher G. A., 2004, J FOOD SCI, V67, P993

[2]

*CAN COUNC AN CAR, 1993, GUID CAR US EXP AN, V1

[3] Pertussis toxin targets airway macrophages to promote Bordetella pertussis infection of the respiratory tract [J].

Carbonetti, Nicholas H. ;

Artamonova, Galina V. ;

Van Rooijen, Nico ;

Ayala, Victor I. .

INFECTION AND IMMUNITY, 2007, 75 (04) :1713-1720

[4]

Cookson AL, 2003, INT J MED MICROBIOL, V292, P547, DOI 10.1078/1438-4221-00235

[5] An in-vitro model for studying the interaction of Escherichia coli O157:H7 and other enteropathogens with bovine primary cell cultures [J].

Dibb-Fuller, MP ;

Best, A ;

Stagg, DA ;

Cooley, WA ;

Woodward, MJ .

JOURNAL OF MEDICAL MICROBIOLOGY, 2001, 50 (09) :759-769

[6] Antiviral activity of Shiga toxin 1: Suppression of bovine leukemia virus-related spontaneous lymphocyte proliferation [J].

Ferens, WA ;

Hovde, CJ .

INFECTION AND IMMUNITY, 2000, 68 (08) :4462-4469

[7] The non-toxic A subunit of Shiga toxin type 1 prevents replication of bovine immunodeficiency virus in infected cells [J].

Ferens, Witold A. ;

Hovde, Carolyn J. .

VIRUS RESEARCH, 2007, 125 (01) :29-41

[8]

Freshney R. I., 2010, Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications

[9] QUANTITATIVE MICROTITER CYTO-TOXICITY ASSAY FOR SHIGELLA TOXIN [J].

GENTRY, MK ;

DALRYMPLE, JM .

JOURNAL OF CLINICAL MICROBIOLOGY, 1980, 12 (03) :361-366

[10] AN INVITRO SYSTEM FOR TESTING BACILLUS-THURINGIENSIS TOXINS - THE LAWN ASSAY [J].

GRINGORTEN, JL ;

WITT, DP ;

MILNE, RE ;

FAST, PG ;

SOHI, SS ;

VANFRANKENHUYZEN, K .

JOURNAL OF INVERTEBRATE PATHOLOGY, 1990, 56 (02) :237-242

← 1 2 →