A rapid, sensitive method for testing the activity of Escherichia coli O157:H7 secreted cytotoxins against epithelial cells from the jejunum and descending colon of cattle

被引：4

作者：

Baines, Danica ^{[1
]}

Masson, Luke ^{[2
]}

McAllister, Tim ^{[1
]}

机构：

[1] Agr & Agri Food Canada, Lethbridge Res Ctr, Lethbridge, AB T1J 4B1, Canada

[2] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada

来源：

CANADIAN JOURNAL OF ANIMAL SCIENCE | 2008年 / 88卷 / 01期

关键词：

Escherichia coli O157 : H7; cattle; intestine; cytotoxins; cytotoxicity;

D O I：

10.4141/CJAS07040

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Current methods for assessing Escherichia coli O157:H7 secreted cytotoxin activity are based on exposing human cell lines for 12 to 72 h and monitoring changes in cell morphology, adherence or enzyme release. These methods, although sensitive, use non-ruminant cell lines that may not represent cattle responses to E. coli O157:H7 secreted cytotoxins. The modified lawn assay used in this study was found to be a simple, fast and sensitive method for assessing cattle epithelial cell susceptibility to E. coli O157:H7 secreted cytotoxins within 4 to 6 h.

引用

页码：51 / 55

页数：5

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