Validation of an isocratic LC method for determination of quercetin and methylquercetin in topical nanoemulsions

被引：30

作者：

Fasolo, Daniel ^{[1
]}

Schwingel, Liege ^{[1
]}

Holzschuh, Maribete ^{[1
]}

Bassani, Valquiria ^{[1
]}

Teixeira, Helder ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Fac Farm, Program Post Grad Ciencias Farmaceut, Porto Alegre, RS, Brazil

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2007年 / 44卷 / 05期

关键词：

quercetin; methylquercetin; nanoemulsions; liquid chromatography; validation;

D O I：

10.1016/j.jpba.2007.04.026

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The aim of this study was to validate an isocratic LC method for the quantification of either quercetin (Q) or methylquercetin (MQ) incorporated in topical nanoemulsions. The analyses were performed at room temperature on a reversed-phase C-18 column using a mobile phase composed of methanol/water (70:30, v/v) and trifluoracetic acid 0.1 % at 0.8 mL min(-1). The detection was carried out on a UV detector at 368 or 354 nm for Q and MQ respectively. The linearity, in the range of 0.15-1.5 mu g/mL, presented a determination coefficient (r(2)) higher than 0.99, calculated by the least square method for both flavonoids. No interferences from the excipients (egg-lecithin or octyldodecanol) were detected. The R.S.D. values for intra- and inter-day precision experiments were lower than 2% for both flavonoids. The recovery ranged from 98.9% to 103.46% for Q and from 98.9% to 102.92% for MQ. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1174 / 1177

页数：4

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