Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member

被引：131

作者：

Matsumoto, A

Okado, A

Fujii, T

Fujii, J

Egashira, M

Niikawa, N

Taniguchi, N

机构：

[1] Osaka Univ, Sch Med, Dept Biochem, Osaka 5650871, Japan

[2] Hokkaido Univ, Sch Med, Dept Cardiovasc Med, Kita Ku, Sapporo, Hokkaido 0608538, Japan

[3] Nagasaki Univ, Sch Med, Dept Human Genet, Nagasaki 8528523, Japan

[4] Nagasaki Univ, Sch Med, Dept Orthopaed, Nagasaki 8528523, Japan

来源：

FEBS LETTERS | 1999年 / 443卷 / 03期

关键词：

thioredoxin peroxidase; secretory form; reactive oxygen species;

D O I：

10.1016/S0014-5793(98)01736-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space. (C) 1999 Federation of European Biochemical Societies.

引用

页码：246 / 250

页数：5