Assessment of the retention properties of poly(vinyl alcohol) stationary phase for lipid class profiling in liquid chromatography

被引：39

作者：

Deschamps, FS ^{[1
]}

Chaminade, P ^{[1
]}

Ferrier, D ^{[1
]}

Baillet, A ^{[1
]}

机构：

[1] Fac Pharm Chatenay Malabry, Grp Chim Analyt Paris Sud, Chim Analyt Lab, F-92296 Chatenay Malabry, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 928卷 / 02期

关键词：

stationary phases; LC; retention behaviour; gradient elution; evaporative light scattering detector; lipids; poly(vinyl alcohol);

D O I：

10.1016/S0021-9673(01)01137-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Potentialities of polymerized vinyl alcohol on silica gel were assessed for class separation of simple lipids, sphingolipids, glyceroglycolipids and phospholipids by high-performance liquid chromatography. A screening of pure solvents in binary gradient elution and a chemometric approach was used to define a rugged two segment linear gradient formed from four solvents for total lipid class separation. Triethylamine and formic acid were added in all mobile phase components for acidic phospholipid separation and evaporative light scattering response enhancement. Simple analytical procedures are described for the analysis of complex lipid materials. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：127 / 137

页数：11