Isolation and characterization of an extracellular haem-binding protein from Pseudomonas aeruginosa that shares function and sequence similarities with the Serratia marcescens HasA haemophore

被引:121
作者
Létoffé, S
Redeker, V
Wandersman, C
机构
[1] Inst Pasteur, Unite Physiol Cellulaire, CNRS, URA 1300, F-75724 Paris 15, France
[2] Ecole Super Phys & Chim Ind, Neurobiol Lab, CNRS, URA 2054, F-75231 Paris, France
关键词
D O I
10.1046/j.1365-2958.1998.00885.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The major mechanism by which bacteria acquire free or haemoglobin-bound haem involves direct binding to specific outer membrane receptors. Serratia marcescens also secretes a haem-binding protein, HasA, which functions as a haemophore that catches haem and shuttles it to a cell surface specific outer membrane receptor, HasR. We report the isolation and characterization of hasAp, a gene from Pseudomonas aeruginosa. HasAp is an iron-regulated extracellular haem-binding protein that shares about 50% identity with HasA. HasAp is required for P. aeruginosa utilization of haemoglobin iron. It can replace HasA for HasR-dependent haemoblobin acquisition in a system reconstituted in Escherichia coli. HasAp, like HasA, lacks a signal peptide and is secreted by an ABC transporter. These findings show that haemophore-dependent haem acquisition is not unique to S. marcescens.
引用
收藏
页码:1223 / 1234
页数:12
相关论文
共 35 条
[1]   Cloning of the Serratia marcescens hasF gene encoding the Has ABC exporter outer membrane component: A TolC analogue [J].
Binet, R ;
Wandersman, C .
MOLECULAR MICROBIOLOGY, 1996, 22 (02) :265-273
[2]  
Braun V., 1991, CRC HDB MICROBIAL IR, P107
[3]   A GENE-CLUSTER INVOLVED IN THE UTILIZATION OF BOTH FREE HEME AND HEME-HEMOPEXIN BY HAEMOPHILUS-INFLUENZAE TYPE-B [J].
COPE, LD ;
YOGEV, R ;
MULLEREBERHARD, U ;
HANSEN, EJ .
JOURNAL OF BACTERIOLOGY, 1995, 177 (10) :2644-2653
[4]  
DELEPELAIRE P, 1989, J BIOL CHEM, V264, P9083
[5]   THE PSEUDOMONAS-FLUORESCENS LIPASE HAS A C-TERMINAL SECRETION SIGNAL AND IS SECRETED BY A 3-COMPONENT BACTERIAL ABC-EXPORTER SYSTEM [J].
DUONG, F ;
SOSCIA, C ;
LAZDUNSKI, A ;
MURGIER, M .
MOLECULAR MICROBIOLOGY, 1994, 11 (06) :1117-1126
[6]   A new type of hemophore-dependent heme acquisition system of Serratia marcescens reconstituted in Escherichia coli [J].
Ghigo, JM ;
Letoffe, S ;
Wandersman, C .
JOURNAL OF BACTERIOLOGY, 1997, 179 (11) :3572-3579
[7]  
GHIGO JM, 1994, J BIOL CHEM, V269, P8979
[8]   Microbial pathogenesis in cystic fibrosis: Mucoid Pseudomonas aeruginosa and Burkholderia cepacia [J].
Govan, JRW ;
Deretic, V .
MICROBIOLOGICAL REVIEWS, 1996, 60 (03) :539-+
[9]   Bacterial transferrin and lactoferrin receptors [J].
GrayOwen, SD ;
Schryvers, AB .
TRENDS IN MICROBIOLOGY, 1996, 4 (05) :185-191
[10]   THE SECRETION GENES OF PSEUDOMONAS-AERUGINOSA ALKALINE PROTEASE ARE FUNCTIONALLY RELATED TO THOSE OF ERWINIA-CHRYSANTHEMI PROTEASES AND ESCHERICHIA-COLI ALPHA-HEMOLYSIN [J].
GUZZO, J ;
DUONG, F ;
WANDERSMAN, C ;
MURGIER, M ;
LAZDUNSKI, A .
MOLECULAR MICROBIOLOGY, 1991, 5 (02) :447-453