Adaptation of an enzymatic polyfructosan assay to clinical practice

被引：30

作者：

Dubourg, Laurence ^{[1
,2
,3
]}

Hadj-Aissa, Aoumeur ^{[1
,2
,3
]}

Ferrier, Bernard ^{[1
,2
]}

机构：

[1] Fac RTH Laennec, INSERM, U820, F-69372 Lyon 08, France

[2] Hop Edouard Herriot, Hosp Civils Lyon, Serv Explorat Fonct Renale & Metabol, F-69437 Lyon, France

[3] Univ Lyon 1, Fac Med Lyon Est, F-69373 Lyon 08, France

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 405卷 / 02期

关键词：

INULIN;

D O I：

10.1016/j.ab.2010.06.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Inulin or polyfructosan clearance is regarded as the most accurate method of assessing the glomerular filtration rate. We propose an enzymatic method of polyfructosan determination based on the hydrolysis of polyfructosan into fructose by inulinase and the elimination of the interfering quantity of glucose by glucose oxidase. This spectrophotometric microplate formatted assay, which demonstrated very good specificity and reproducibility (within-run precision <1% and between-run precision <3.5%), is cheap and simple to perform and can be used by all analytical laboratories and in all clinical conditions. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：266 / 268

页数：3

共 9 条

[1]

ALOIA JF, 1973, J LAB CLIN MED, V82, P809

[2]

GALLI A, 1965, ANN BIOL CLIN-PARIS, V23, P931

[3] Evaluation of a new automated, enzymatic inulin assay using d-fructose dehydrogenase [J].

Kimata, Shinsuke ;

Mizuguchi, Katsuhiko ;

Hattori, Shizuo ;

Teshima, Shinichi ;

Orita, Yoshimasa .

CLINICAL AND EXPERIMENTAL NEPHROLOGY, 2009, 13 (04) :341-349

[4] FULLY ENZYMATIC INULIN DETERMINATION IN SMALL VOLUME SAMPLES WITHOUT DEPROTEINIZATION [J].

KUEHNLE, HF ;

SCHMIDT, FH ;

VONDAHL, K .

NEPHRON, 1992, 62 (01) :104-107

[5]

SCHREINER GE, 1950, P SOC EXP BIOL MED, V74, P117

[6]

SOPER CPR, 1995, EUR J CLIN CHEM CLIN, V33, P497

[7] A NEW ENZYMATIC METHOD FOR THE DETERMINATION OF INULIN [J].

SUGITA, O ;

TOMIYAMA, Y ;

MATSUTO, T ;

OKADA, M ;

GEJYO, F ;

ARAKAWA, M ;

TAKAHASHI, S ;

WATAZU, Y ;

KANEDA, N .

ANNALS OF CLINICAL BIOCHEMISTRY, 1995, 32 :561-565

[8]

SUMMERFIELD AL, 1993, CLIN CHEM, V39, P2333

[9]

WHITE RP, 1954, J LAB CLIN MED, V43, P475

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