Morphology and structure of electrospun mats from regenerated silk fibroin aqueous solutions with adjusting pH

被引:63
作者
Zhu, Jingxin [1 ]
Shao, Huili [1 ]
Hu, Xuechao [1 ]
机构
[1] Donghua Univ, Coll Mat Sci & Engn, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 200051, Peoples R China
关键词
electrospinning; silk fibroin; aqueous solutions; morphology; structure;
D O I
10.1016/j.ijbiomac.2007.06.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this paper, regenerated silk fibroin (SF) aqueous solutions were adjusted to a pH of 6.9 by mimicing the condition in the posterior division of silkworm's gland and theological behavior of solutions was investigated. The electrospinning technique was used to prepare fibers, and non-woven mats of regenerated B. mori silk fibroin were successfully obtained. The effects of electrospinning parameters on the morphology and diameter of regenerated silk fibers were investigated by orthogonal design. Statistical analysis showed that voltage, the concentration of regenerated SF solutions and the distance between tip and collection plate were the most dominant parameters to fiber morphology, diameter and diameter distribution, respectively. An optimal electrospinning condition was obtained in producing uniform cylindrical fibers with an average diameter of 1300 nm. It was as follows: the concentration 30%, voltage 40 kV, distance 20 cm. The structure of electrospun mats was characterized by Raman spectroscopy (RS), wide-angle X-ray diffraction (WAXD) and modulated differential scanning calorimetry (MDSC). It was found that electrospun mats were predominantly random coil/silk I structure, and the transition to silk II (beta-sheet) rich structure should be further explored. (C) 2007 Elsevier B.V.. All rights reserved.
引用
收藏
页码:469 / 474
页数:6
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