Structural studies of oligonucleotides containing G-quadruplex motifs using AFM

被引：41

作者：

Costa, LT

Kerkmann, M

Hartmann, G

Endres, S

Bisch, PM

Heckl, WM

Thalhammer, S ^{[1
]}

机构：

[1] LMU, Dept Geo & Environm Sci, D-80333 Munich, Germany

[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21949900 Rio de Janeiro, Brazil

[3] Univ Fed Rio Grande do Sul, Ctr Biotecnol, BR-91501970 Porto Alegre, RS, Brazil

[4] LMU, Dept Internal Med, Div Clin Pharmacol, D-80333 Munich, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 313卷 / 04期

关键词：

AFM; palindromic sequence oligonucleotides; G-wire; self-assembled DNA network; nanowire;

D O I：

10.1016/j.bbrc.2003.12.041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

G-quadruplex DNAs are cyclic arrays of four guanine bases binding by Hoogsteen hydrogen bonds, found in the telomeric regions of chromosomes and in transcriptional regulatory regions of several important oncogenes. Here, we used high resolution atomic force microscopy (AFM) to observe a specific guanine (G) tetrad mediated complex formation of oligonucleotides containing a G-quadruplex motifs (G-ODN) combined with a palindromic sequence under physiological extracellular conditions. These oligonucleotides have been investigated in correlation to their immunostimulatory effects. We observed structural dependence on ion concentration and G-ODN concentration, where high concentration self-assembled DNA networks were formed. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：1065 / 1072

页数：8

共 33 条

[31] Determination of the number and location of the manganese binding sites of DNA quadruplexes in solution by EPR and NMR in the presence and absence of thrombin [J].