Separation of bacteria by capillary electrophoresis

被引：76

作者：

Buszewski, B

Szumski, M

Klodzinska, E

Dahm, H

机构：

[1] Nicholas Copernicus Univ, Fac Chem, Dept Environm Chem & Ecoanalyt, PL-87100 Torun, Poland

[2] Nicholas Copernicus Univ, Fac Biol & Earth Sci, Dept Microbiol, PL-87100 Torun, Poland

来源：

JOURNAL OF SEPARATION SCIENCE | 2003年 / 26卷 / 11期

关键词：

capillary electrophoresis; bacteria; electroosmotic flow; electrophoretic mobility;

D O I：

10.1002/jssc.200301442

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Differences in the surface charges of bacteria can be exploited for their separation by capillary electrophoresis. Because of their low electrophoretic mobility, the separation is not always easy to perform, especially in the presence of the electroosmotic flow. Elimination of electroosmotic flow by capillary wall modification with gamma-(trimethoxysilyl)propyl methacrylate followed by acrylamide bonding permits separation over a distance of 8.5 cm.

引用

页码：1045 / 1049

页数：5

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