Eyes Wide Open: A Critical Review of Sphere-Formation as an Assay for Stem Cells

被引：630

作者：

Pastrana, Erika ^{[1
,2
,3
]}

Silva-Vargas, Violeta ^{[1
,2
,3
]}

Doetsch, Fiona ^{[1
,2
,3
]}

机构：

[1] Columbia Univ, Med Ctr, Dept Pathol & Cell Biol, New York, NY 10032 USA

[2] Columbia Univ, Med Ctr, Dept Neurol, New York, NY 10032 USA

[3] Columbia Univ, Med Ctr, Ctr Motor Neuron Biol & Dis, Columbia Stem Cell Initiat,Dept Neurosci, New York, NY 10032 USA

来源：

CELL STEM CELL | 2011年 / 8卷 / 05期

关键词：

ADULT MAMMALIAN BRAIN; CENTRAL-NERVOUS-SYSTEM; NEURAL STEM/PROGENITOR CELLS; SUBVENTRICULAR ZONE; PROGENITOR CELLS; SELF-RENEWAL; OLFACTORY-BULB; IN-VITRO; SUBEPENDYMAL ZONE; EPENDYMAL CELLS;

D O I：

10.1016/j.stem.2011.04.007

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Sphere-forming assays have been widely used to retrospectively identify stem cells based on their reported capacity to evaluate self-renewal and differentiation at the single-cell level in vitro. The discovery of markers that allow the prospective isolation of stem cells and their progeny from their in vivo niche allows the functional properties of purified populations to be defined. We provide a historical perspective of the evolution of the neurosphere assay and highlight limitations in the use of sphere-forming assays in the context of neurospheres. We discuss theoretical and technical considerations of experimental design and interpretation that surround the use of this assay with any tissue.

引用

收藏

页码：486 / 498

页数：13

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