Selective coactivation of estrogen-dependent transcription by CITED1 CBP/p300-binding protein

被引:106
作者
Yahata, T
Shao, WL
Endoh, H
Hur, JY
Coser, KR
Sun, HP
Ueda, Y
Kato, S
Isselbacher, KJ
Brown, M
Shioda, T [1 ]
机构
[1] Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
[2] Dana Farber Canc Inst, Dept Adult Oncol, Boston, MA 02115 USA
[3] Yamanouchi Pharmaceut, Mol Med Labs, Tsukuba, Ibaraki 305, Japan
[4] Univ Tokyo, Inst Mol & Cellular Biosci, Tokyo 113, Japan
关键词
CITED protein; CBP/p300; estrogen receptor; nuclear receptor; transcriptional coactivator; transforming growth factor-alpha;
D O I
10.1101/gad.906301
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
CITED1, a CBP/p300.binding nuclear protein that does not bind directly to DNA, is a transcriptional coregulator. Here, we show evidence that CITED1 functions as a selective coactivator for estrogen-dependent transcription. When transfected, CITED1 enhanced transcriptional activation by the ligand-binding/AF2 domain of both estrogen receptor-alpha (ER alpha) and ER beta in an estrogen-dependent manner, but it affected transcriptional activities of other nuclear receptors only marginally. CITED1 bound directly to ER alpha in an estrogen-dependent manner through its transactivating domain, and this binding activity was separable from its p300-binding activity. CITED1 was strongly expressed in nulliparous mouse mammary epithelial cells and, when expressed in ER-positive MCF-7 breast cancer cells by transduction, exogenous CITED1 enhanced sensitivity of MCF-7 cells to estrogen, stabilizing the estrogen-dependent interaction between p300 and ER alpha. The estrogen-induced expression of the transforming growth factor-alpha (TGF-alpha) mRNA transcript was enhanced in the CITED1-expressing MCF-7 cells, whereas estrogen-induced expression of the mRNA transcripts for progesterone receptor or pS2 was not affected. Chromatin immunoprecipitation assay revealed that endogenous CITED1 is recruited to the chromosomal TGF-alpha promoter in MCF-7 cells in an estrogen-dependent manner but not to the pS2 promoter. These results suggest that CITED1 may play roles in regulation of estrogen sensitivity in a gene-specific manner.
引用
收藏
页码:2598 / 2612
页数:15
相关论文
共 41 条
  • [21] p300 and estrogen receptor cooperatively activate transcription via differential enhancement of initiation and reinitiation
    Kraus, WL
    Kadonaga, JT
    [J]. GENES & DEVELOPMENT, 1998, 12 (03) : 331 - 342
  • [22] Li DS, 1999, MOL CELL BIOL, V19, P7191
  • [23] Regulation of expression of MSG1 melanocyte-specific nuclear protein in human melanocytes and melanoma cells
    Li, HC
    Ahmed, NU
    Fenner, MH
    Ueda, M
    Isselbacher, KJ
    Shioda, T
    [J]. EXPERIMENTAL CELL RESEARCH, 1998, 242 (02) : 478 - 486
  • [24] Effect of pituitary and ovarian hormones on human melanocytes in vitro
    Maeda, K
    Naganuma, M
    Fukuda, M
    Matsunaga, J
    Tomita, Y
    [J]. PIGMENT CELL RESEARCH, 1996, 9 (04): : 204 - 212
  • [25] Estrogen receptor affinity and location of consensus and imperfect estrogen response elements influence transcription activation of simplified promoters
    Nardulli, AM
    Romine, LE
    Carpo, C
    Greene, GL
    Rainish, B
    [J]. MOLECULAR ENDOCRINOLOGY, 1996, 10 (06) : 694 - 704
  • [26] MCF-7 BREAST-CANCER CELLS GROWN AS MULTICELLULAR SPHEROIDS INVITRO - EFFECT OF 17-BETA-ESTRADIOL
    OLEA, N
    VILLALOBOS, M
    DEALMODOVAR, JMR
    PEDRAZA, V
    [J]. INTERNATIONAL JOURNAL OF CANCER, 1992, 50 (01) : 112 - 117
  • [27] Planas-Silva MD, 2001, CANCER RES, V61, P3858
  • [28] HUMAN MELANOCYTES AS A TARGET TISSUE FOR HORMONES - INVITRO STUDIES WITH 1-ALPHA-25, DIHYDROXYVITAMIN-D3, ALPHA-MELANOCYTE STIMULATING HORMONE, AND BETA-ESTRADIOL
    RANSON, M
    POSEN, S
    MASON, RS
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1988, 91 (06) : 593 - 598
  • [29] Expression analysis of the chicken homologue of CITED2 during early stages of embryonic development
    Schlange, T
    Andrée, B
    Arnold, HH
    Brand, T
    [J]. MECHANISMS OF DEVELOPMENT, 2000, 98 (1-2) : 157 - 160
  • [30] Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription
    Shang, YF
    Hu, X
    DiRenzo, J
    Lazar, MA
    Brown, M
    [J]. CELL, 2000, 103 (06) : 843 - 852