Selective coactivation of estrogen-dependent transcription by CITED1 CBP/p300-binding protein
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作者:
Yahata, T
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机构:Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
Yahata, T
Shao, WL
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机构:Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
Shao, WL
Endoh, H
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机构:Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
Endoh, H
Hur, JY
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机构:Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
Hur, JY
Coser, KR
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机构:Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
Coser, KR
Sun, HP
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机构:Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
Sun, HP
Ueda, Y
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机构:Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
Ueda, Y
Kato, S
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机构:Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
Kato, S
Isselbacher, KJ
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机构:Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
Isselbacher, KJ
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Brown, M
Shioda, T
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Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USAMassachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
Shioda, T
[1
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机构:
[1] Massachusetts Gen Hosp, Ctr Canc, Lab Tumor Biol, Charlestown, MA 02129 USA
[2] Dana Farber Canc Inst, Dept Adult Oncol, Boston, MA 02115 USA
[3] Yamanouchi Pharmaceut, Mol Med Labs, Tsukuba, Ibaraki 305, Japan
[4] Univ Tokyo, Inst Mol & Cellular Biosci, Tokyo 113, Japan
CITED1, a CBP/p300.binding nuclear protein that does not bind directly to DNA, is a transcriptional coregulator. Here, we show evidence that CITED1 functions as a selective coactivator for estrogen-dependent transcription. When transfected, CITED1 enhanced transcriptional activation by the ligand-binding/AF2 domain of both estrogen receptor-alpha (ER alpha) and ER beta in an estrogen-dependent manner, but it affected transcriptional activities of other nuclear receptors only marginally. CITED1 bound directly to ER alpha in an estrogen-dependent manner through its transactivating domain, and this binding activity was separable from its p300-binding activity. CITED1 was strongly expressed in nulliparous mouse mammary epithelial cells and, when expressed in ER-positive MCF-7 breast cancer cells by transduction, exogenous CITED1 enhanced sensitivity of MCF-7 cells to estrogen, stabilizing the estrogen-dependent interaction between p300 and ER alpha. The estrogen-induced expression of the transforming growth factor-alpha (TGF-alpha) mRNA transcript was enhanced in the CITED1-expressing MCF-7 cells, whereas estrogen-induced expression of the mRNA transcripts for progesterone receptor or pS2 was not affected. Chromatin immunoprecipitation assay revealed that endogenous CITED1 is recruited to the chromosomal TGF-alpha promoter in MCF-7 cells in an estrogen-dependent manner but not to the pS2 promoter. These results suggest that CITED1 may play roles in regulation of estrogen sensitivity in a gene-specific manner.
机构:Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
Li, HC
Ahmed, NU
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机构:Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
Ahmed, NU
Fenner, MH
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机构:Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
Fenner, MH
Ueda, M
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机构:Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
Ueda, M
Isselbacher, KJ
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机构:Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
Isselbacher, KJ
Shioda, T
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Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USAMassachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
机构:Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
Li, HC
Ahmed, NU
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机构:Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
Ahmed, NU
Fenner, MH
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机构:Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
Fenner, MH
Ueda, M
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机构:Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
Ueda, M
Isselbacher, KJ
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机构:Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA
Isselbacher, KJ
Shioda, T
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机构:
Massachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USAMassachusetts Gen Hosp E, MGH Canc Ctr, Lab Tumor Biol, Charlestown, MA 02129 USA