Toxicarioside A inhibits SGC-7901 proliferation, migration and invasion via NF-κB/bFGF signaling

被引:24
作者
Guo, Jun-Li [2 ]
Zheng, Shao-Jiang [2 ]
Li, Yue-Nan [2 ]
Jie, Wei [3 ]
Hao, Xin-Bao [2 ]
Li, Tian-Fa [2 ]
Xia, Li-Ping [2 ]
Mei, Wen-Li [1 ]
Huang, Feng-Ying [2 ]
Kong, Yue-Qiong [2 ]
He, Qi-Yi [2 ]
Yang, Kun [2 ]
Tan, Guang-Hong [2 ]
Dai, Hao-Fu [1 ]
机构
[1] Chinese Acad Trop Agr Sci, Minist Agr, Inst Trop Biosci & Biotechnol, Key Lab Trop Crop Biotechnol, Haikou 571101, Hainan Province, Peoples R China
[2] Hainan Med Coll, Affiliated Hosp, Canc Res Inst, Key Lab Trop Med, Haikou 571199, Hainan Province, Peoples R China
[3] Guangdong Med Coll, Dept Pathol, Zhanjiang 524023, Guangdong, Peoples R China
关键词
Anti-migration; Anti-proliferation; Basic fibroblast growth factor; Gastric cancer; Nuclear factor-kappa B; Toxicarioside A; FIBROBLAST-GROWTH-FACTOR; LUNG-CANCER CELLS; CARDIAC-GLYCOSIDES; ANTIARIS-TOXICARIA; TRANSCRIPTION FACTOR; THERAPEUTIC TARGET; PROSTATE-CANCER; GASTRIC-CANCER; APOPTOSIS; ADENOCARCINOMA;
D O I
10.3748/wjg.v18.i14.1602
中图分类号
R57 [消化系及腹部疾病];
学科分类号
100201 [内科学];
摘要
AIM: To investigate the inhibitory role of toxicarioside A on the gastric cancer cell line human gastric cancer cell line (SGC-7901) and determine the underlying molecular mechanism. METHODS: After SGC-7901 cells were treated with toxicarioside A at various concentrations (0.5, 1.5, 4.5, 9.0 mu g/mL) for 24 h or 48 h, cell viability was determined by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, and the motility and invasion of tumor cells were assessed by the Transwell chamber assay. Immunofluorescence staining, reverse transcription polymerase chain reaction and Western blotting were performed to detect the expression of basic fibroblast growth factor (bFGF) and fibroblast growth factor receptor-1 (FGFR1), and nuclear factor-kappa B (NF-kappa B) activation was examined by electrophoretic mobility shift assay. RESULTS: The results showed that toxicarioside A was capable of reducing cell viability, inhibiting cell growth, and suppressing cell migration and invasion activities in a time- and dose-dependent manner in SGC-7901 cells. Further analysis revealed that not only the expression of bFGF and its high-affinity receptor FGFR1 but also the NF-kappa B-DNA binding activity were effectively blocked by toxicarioside A in a dose-dependent manner compared with the control group (P < 0.05 or P < 0.01). Interestingly, application of the NF-kappa B specific inhibitor, pyrrolidinedithiocarbamate (PDTC), to SGC-7901 cells significantly potentized the toxicarioside A-induced down-regulation of bFGF compared with the control group (P < 0.05). CONCLUSION: These findings suggest that toxicarioside A has an anti-gastric cancer activity and this effect may be achieved partly through down-regulation of NF-kappa B and bFGF/FGFR1 signaling. (C) 2012 Baishideng. All rights reserved.
引用
收藏
页码:1602 / 1609
页数:8
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