The genomic organization of the gene encoding the vanilloid receptor: Evidence for multiple splice variants

被引：39

作者：

Xue, Q ^{[1
]}

Yu, Y ^{[1
]}

Trilk, SL ^{[1
]}

Jong, BE ^{[1
]}

Schumacher, MA ^{[1
]}

机构：

[1] Univ Calif San Francisco, San Francisco Dept Anesthesia & Perioperat Care, San Francisco, CA 94143 USA

来源：

GENOMICS | 2001年 / 76卷 / 1-3期

关键词：

capsaicin; ion channel; pain; splice variant; stretch-inactivated channel (SIC); vanilloid receptor; VR1; VR.5'sv; VROAC;

D O I：

10.1006/geno.2001.6582

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Vanilloid receptor subtype-1 (VR1) is a nonselective cation channel that is expressed in sensory neurons and is activated by multiple noxious stimuli. Rat Vr1, stretch-inactivated channel (SIC), and vanilloid receptor 5 ' splice variant (VR.5 ' sv) have been hypothesized to be derived from a common VR gene. Characterization of the genomic structure encoding the 5 ' portion of rat Vr1 confirmed that VR.5 ' sv is derived from the VR gene; however, SIC seemed to be derived from two related but independent genes. We also deduced the genomic organization of the human gene VR1. Comparative studies of rat and human VR genes showed substantial conservation in genomic organization. The splice site flanking exon-intron 7 in rat and human VR1 diverged from the expected consensus sequence; this may help to explain the skipping of exon 7 within VR.5 ' sv and other VR splice variants.

引用

页码：14 / 20

页数：7

共 30 条

[1] SEQUENCE REQUIREMENTS FOR SPLICING OF HIGHER EUKARYOTIC NUCLEAR PRE-MESSENGER-RNA [J].