Engineering molecular recognition in alkane oxidation catalysed by cytochrome P450cam

被引：20

作者：

Stevenson, JA ^{[1
]}

Bearpark, JK ^{[1
]}

Wong, LL ^{[1
]}

机构：

[1] Univ Oxford, Inorgan Chem Lab, Dept Chem, Oxford OX1 3QR, England

来源：

NEW JOURNAL OF CHEMISTRY | 1998年 / 22卷 / 06期

关键词：

D O I：

10.1039/a801637b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The activity, selectivity and coupling efficiency of alkane oxidation by the monooxygenase enzyme cytochrome P450(cam) can be enhanced by engineering the active site topology to accommodate different alkanes: reducing the active site volume by the Y96F-V247L double mutations resulted in four-fold higher activity for the oxidation of hexane (1) than 3-methylpentane (2) while the larger active site of the Y96A-V247A double mutant gave rise to a two-fold preference for 2 over 1.

引用

页码：551 / 552

页数：2

共 12 条

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