The catalytic activity of cytochrome P450(cam) towards styrene oxidation is increased by site-specific mutagenesis

被引：68

作者：

Nickerson, DP ^{[1
]}

HarfordCross, CF ^{[1
]}

Fulcher, SR ^{[1
]}

Wong, LL ^{[1
]}

机构：

[1] UNIV OXFORD, INORGAN CHEM LAB, OXFORD OX1 3QR, ENGLAND

来源：

FEBS LETTERS | 1997年 / 405卷 / 02期

关键词：

monooxygenase; P450; mutagenesis; styrene; epoxidation; stereoselectivity;

D O I：

10.1016/S0014-5793(97)00174-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The styrene oxidation activity of cytochrome P450(cam) has been greatly improved by rational protein engineering. Compared to the wild-type enzyme, the active-site mutants Y96A and Y96F bound styrene more tightly, consumed NADH more rapidly, and were more efficient at utilising reducing equivalents for product formation. Styrene oxide formation rates were enhanced 9-fold in the Y96A mutant relative to wild-type, and 25-fold in the Y96F mutant, thus demonstrating the effectiveness of active-site redesign in improving the activity of a haem monooxygenase towards an unnatural substrate. (C) 1997 Federation of European Biochemical Societies.

引用

页码：153 / 156

页数：4

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