The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems

被引：41

作者：

Kratasyuk, VA ^{[1
]}

Esimbekova, EN ^{[1
]}

Gladyshev, MI ^{[1
]}

Khromichek, EB ^{[1
]}

Kuznetsov, AM ^{[1
]}

Ivanova, EA ^{[1
]}

机构：

[1] Krasnoyarsk State Agr Univ, Krasnoyarsk 660049, Russia

来源：

CHEMOSPHERE | 2001年 / 42卷 / 08期

关键词：

bioluminescence; bacterial luciferase; alcohol dehydrogenase; trypsin; water toxicity; blooming; pollution;

D O I：

10.1016/S0045-6535(00)00177-6

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductaseluciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and "bloom" of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the "bloom" of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：909 / 915

页数：7