Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Identification of Beta-Hemolytic Streptococci

被引：74

作者：

Cherkaoui, Abdessalam ^{[1
]}

Emonet, Stephane ^{[1
]}

Fernandez, Jose ^{[1
]}

Schorderet, Didier ^{[1
]}

Schrenzel, Jacques ^{[1
,2
]}

机构：

[1] Univ Geneva Hosp HUG, Infect Dis Serv, Bacteriol Lab, CH-1211 Geneva 14, Switzerland

[2] Univ Geneva Hosp HUG, Infect Dis Serv, Genom Res Lab, Dept Internal Med, CH-1211 Geneva 14, Switzerland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 08期

关键词：

ROUTINE IDENTIFICATION; BACTERIA; MICROBIOLOGY;

D O I：

10.1128/JCM.00240-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study was undertaken to evaluate matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of beta-hemolytic streptococci. We compared Bruker Biotyper 2.0 with Vitek2 coupled to the agglutination test. MALDI-TOF MS analysis of 386 beta-hemolytic streptococcal isolates yielded high-confidence identification to the species level for all 386 isolates. The Vitek2 gave high-confidence identification to the species level for 88% of Streptococcus agalactiae isolates (n = 269/306), 92% of Streptococcus pyogenes isolates (n = 48/52), and 39% of isolates of Streptococcus dysgalactiae serogroups C and G (n = 11/28).

引用

页码：3004 / 3005

页数：2

共 15 条

[1] Identification of Campylobacter species and related organisms by matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry [J].