A tightly regulated molecular motor based upon T7 RNA polymerase

被引：24

作者：

Pomerantz, RT

Ramjit, R

Gueroui, Z

Place, C

Anikin, M

Leuba, S

Zlatanova, J

McAllister, WT

机构：

[1] Suny Downstate Med Ctr, Dept Microbiol & Immunol, Brooklyn, NY 11203 USA

[2] Suny Downstate Med Ctr, Grad Program Mol & Cellular Biol, Brooklyn, NY 11203 USA

[3] Ecole Normale Super Lyon, CNRS, UMR 5672, Phys Lab, F-69007 Lyon, France

[4] Polytech Univ, Dept Chem & Biol Sci, MetroTech Ctr 6, Brooklyn, NY 11201 USA

[5] Univ Rennes 1, Lab Struct & Proprietes Mat, UMR 6626, F-35042 Rennes, France

[6] Univ Pittsburgh, Sch Med, Dept Cell Biol & Physiol, Hillman Canc Ctr UPCI, Pittsburgh, PA 15213 USA

[7] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA

来源：

NANO LETTERS | 2005年 / 5卷 / 09期

关键词：

D O I：

10.1021/nl0509125

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Controlled movement of materials or molecules within the nanometer range is essential in many applications of nanotechnology. Here we report the capture, movement, and release of cargo molecules along DNA by a modified form of T7 RNA polymerase (RNAP) in a manner that is controlled by the sequence of the DNA. Using single-molecule methods, we visualize the assembly and manipulation of nanodevices and the ability to harness rotary and linear forces of the RNAP motor.

引用

页码：1698 / 1703

页数：6

共 27 条

[1] SIGNAL-MEDIATED IMPORT OF BACTERIOPHAGE-T7 RNA-POLYMERASE INTO THE SACCHAROMYCES-CEREVISIAE NUCLEUS AND SPECIFIC TRANSCRIPTION OF TARGET GENES [J].