Pseudolaric acid B induces apoptosis via activation of c-Jun N-terminal kinase and caspase-3 in HeLa cells

被引：41

作者：

Gong, XF

Wang, MW

Wu, Z

Tashiro, S

Onodera, S

Ikejima, T ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, China Japan Inst Med Pharmaceut, Shenyang 110016, Peoples R China

[2] Shenyang Pharmaceut Univ, Dept Pharmacol, Shenyang 110016, Peoples R China

[3] Showa Pharmaceut Univ, Dept Clin & Biomed Sci, Tokyo 1948543, Japan

来源：

EXPERIMENTAL AND MOLECULAR MEDICINE | 2004年 / 36卷 / 06期

关键词：

apoptosis; caspase; HeLa; MAPK; pseudolaric acid B;

D O I：

10.1038/emm.2004.70

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pseudolaric acid B was isolated from Pseudolarix kaempferi Gordon (Pinaceae) and was evaluated for the anti-cancer effect in HeLa cells. We observed that pseudolaric acid B inhibited cell proliferation and induced apoptosis in a time- and dose-dependent manner. HeLa cells treated with pseudolaric acid B showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. JNK inhibitor, SP600125, markedly inhibited pseudolaric acid B-induced cell death. In addition, Bcl-2 expression was down-regulated while Bax protein level was up-regulated. Caspase-3 inhibitor, z-DEVD-fmk, partially blocked pseudolaric acid B-induced cell death, and the expression of two classical caspase substrates, PARP and ICAD, were both decreased in a time-dependent manner, indicative of downstream caspase activation.

引用

页码：551 / 556

页数：6

共 28 条

[21] Mitogen-activated protein kinase cascades and regulation of gene expression [J].