The atpIBEXF operon coding for the F0 sector of the ATP synthase from the purple nonsulfur photosynthetic bacterium Rhodobacter capsulatus

被引：15

作者：

Borghese, R ^{[1
]}

Turina, P ^{[1
]}

Lambertini, L ^{[1
]}

Melandri, BA ^{[1
]}

机构：

[1] Univ Bologna, Dipartimento Biol, I-40126 Bologna, Italy

来源：

ARCHIVES OF MICROBIOLOGY | 1998年 / 170卷 / 05期

关键词：

F0F1 ATP synthase; F0F1; ATPase; Rhodobacter capsulatus; F-0; operon; gene cloning; promoter; GTA; photosynthesis; dimethylsulfoxide respiration;

D O I：

10.1007/s002030050657

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The atpIBEXF operon coding for the F-0 sector of the ATP synthase from Rhodobacter capsulatus was cloned and sequenced. The genes for the five subunits were present in the order: atpI (subunit I), atpB (subunit a), atpE (subunit c), atpX (subunit b'), and atpF (subunit b). The transcription initiation site was defined by primer-extension analysis. A duplicated and divergent copy of the b subunit gene (subunit b') was present. This duplication is found only in photosynthetic prokaryotes and in plant chloroplasts, F-0 deletion mutants formed tiny colonies during anaerobic growth in the dark but could not sustain continuous growth. Based on the results of the present work, we conclude that a functioning ATP synthase is essential for normal growth under all conditions tested.

引用

页码：385 / 388

页数：4

共 32 条

[1] STRUCTURE AT 2.8-ANGSTROM RESOLUTION OF F1-ATPASE FROM BOVINE HEART-MITOCHONDRIA [J].