A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands

被引：195

作者：

Stark, Alexander ^{[1
,2
,3
]}

Bushati, Natascha ^{[4
]}

Jan, Calvin H. ^{[5
,6
]}

Kheradpour, Pouya ^{[1
,2
,3
]}

Hodges, Emily ^{[7
,8
]}

Brennecke, Julius ^{[7
,8
]}

Bartel, David P. ^{[5
,6
]}

Cohen, Stephen M. ^{[4
]}

Kellis, Manolis ^{[1
,2
]}

机构：

[1] MIT, Broad Inst, Cambridge, MA 02141 USA

[2] Harvard Univ, Cambridge, MA 02141 USA

[3] MIT, Comp Sci & Artificial Intelligence Lab, Cambridge, MA 02139 USA

[4] European Mol Biol Lab, D-69117 Heidelberg, Germany

[5] MIT, Dept Biol, Howard Hughes Med Inst, Cambridge, MA 02139 USA

[6] MIT, Dept Biol, Whitehead Inst Biomed Res, Cambridge, MA 02139 USA

[7] Cold Spring Harbor Lab, Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA

[8] Cold Spring Harbor Lab, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA

来源：

GENES & DEVELOPMENT | 2008年 / 22卷 / 01期

关键词：

Drosophila; miR-iab-4; Hox; antisense miRNAs;

D O I：

10.1101/gad.1613108

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

MicroRNAs (miRNAs) are similar to 22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression. Here, we report that the miRNA iab-4 locus in the Drosophila Hox cluster is transcribed convergently from both DNA strands, giving rise to two distinct functional miRNAs. Both sense and antisense miRNA products target neighboring Hox genes via highly conserved sites, leading to homeotic transformations when ectopically expressed. We also report sense/antisense miRNAs in mouse and find antisense transcripts close to many miRNAs in both flies and mammals, suggesting that additional sense/antisense pairs exist.

引用

页码：8 / 13

页数：6

共 39 条

[1] Characterization of the intergenic RNA profile at abdominal-A and Abdominal-B in the Drosophila bithorax complex
Bae, E
Calhoun, VC
Levine, M
Lewis, EB
Drewell, RA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (26) : 16847 - 16852
[2] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004)
Bartel, David P.
[J]. CELL, 2007, 131 (04) : 11 - 29
[3] BOULET AM, 1991, DEVELOPMENT, V111, P393
[4] Principles of MicroRNA-target recognition
Brennecke, J
Stark, A
Russell, RB
Cohen, SM
[J]. PLOS BIOLOGY, 2005, 3 (03): : 404 - 418
[5] Brennecke J, 2007, CELL, V128, P1089, DOI 10.1016/j.cell.2007.01.043
[6] Evolution of genes and genomes on the Drosophila phylogeny
Clark, Andrew G.
Eisen, Michael B.
Smith, Douglas R.
Bergman, Casey M.
Oliver, Brian
Markow, Therese A.
Kaufman, Thomas C.
Kellis, Manolis
Gelbart, William
Iyer, Venky N.
Pollard, Daniel A.
Sackton, Timothy B.
Larracuente, Amanda M.
Singh, Nadia D.
Abad, Jose P.
Abt, Dawn N.
Adryan, Boris
Aguade, Montserrat
Akashi, Hiroshi
Anderson, Wyatt W.
Aquadro, Charles F.
Ardell, David H.
Arguello, Roman
Artieri, Carlo G.
Barbash, Daniel A.
Barker, Daniel
Barsanti, Paolo
Batterham, Phil
Batzoglou, Serafim
Begun, Dave
Bhutkar, Arjun
Blanco, Enrico
Bosak, Stephanie A.
Bradley, Robert K.
Brand, Adrianne D.
Brent, Michael R.
Brooks, Angela N.
Brown, Randall H.
Butlin, Roger K.
Caggese, Corrado
Calvi, Brian R.
de Carvalho, A. Bernardo
Caspi, Anat
Castrezana, Sergio
Celniker, Susan E.
Chang, Jean L.
Chapple, Charles
Chatterji, Sourav
Chinwalla, Asif
Civetta, Alberto
[J]. NATURE, 2007, 450 (7167) : 203 - 218
[7] CHARACTERIZATION OF 2 RNAS TRANSCRIBED FROM THE CIS-REGULATORY REGION OF THE ABD-A DOMAIN WITHIN THE DROSOPHILA BITHORAX COMPLEX
CUMBERLEDGE, S
ZARATZIAN, A
SAKONJU, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (09) : 3259 - 3263
[8] Vertebrate Hox gene regulation:: clustering and/or colinearity?
Duboule, D
[J]. CURRENT OPINION IN GENETICS & DEVELOPMENT, 1998, 8 (05) : 514 - 518
[9] The widespread impact of mammalian microRNAs on mRNA repression and evolution
Farh, KKH
Grimson, A
Jan, C
Lewis, BP
Johnston, WK
Lim, LP
Burge, CB
Bartel, DP
[J]. SCIENCE, 2005, 310 (5755) : 1817 - 1821
[10] miRBase: microRNA sequences, targets and gene nomenclature
Griffiths-Jones, Sam
Grocock, Russell J.
van Dongen, Stijn
Bateman, Alex
Enright, Anton J.
[J]. NUCLEIC ACIDS RESEARCH, 2006, 34 : D140 - D144

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