Applicability of a rapid duplex real-time PCR assay for speciation of Campylobacter jejuni and Campylobacter coli directly from culture plates

被引：116

作者：

Best, EL

Powell, EJ

Swift, C

Grant, KA

Frost, JA

机构：

[1] Specialist & Reference Microbiol Div, Campylobacter Reference Unit, Lab Enter Pathogens, Hlth Protect Agcy, London NW9 5HT, England

[2] Specialist & Reference Microbiol Div, Food Pathogen Reference Unit, Food Safety Microbiol Lab, Hlth Protect Agcy, London NW9 5HT, England

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 229卷 / 02期

关键词：

real-time polymerase chain reaction; ABI prism 7700; Campylobacter speciation;

D O I：

10.1016/S0378-1097(03)00845-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid duplex real-time polymerase chain reaction (PCR) assay for speciation of Campylobacter jejuni and Campylobacter coli using the ABI Prism 7700 sequence detection system (Applied Biosystems) was developed based on two of the genes used in a conventional multiplex PCR. A rapid turnaround time of 3 h was achieved with the use of boiled cell lysates. Applicability of the assay was tested with 6015 random campylobacter strains referred to the Campylobacter Reference Unit, with 97.6% being identified as either C. jejuni or C. coli by this technique. Rapidity, combined with specificity and sensitivity, makes this method for routine campylobacter speciation attractive to any laboratory with a Taqman system. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：237 / 241

页数：5

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