Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli

被引：303

作者：

Denis, M ^{[1
]}

Soumet, C ^{[1
]}

Rivoal, K ^{[1
]}

Ermel, G ^{[1
]}

Blivet, D ^{[1
]}

Salvat, G ^{[1
]}

Colin, P ^{[1
]}

机构：

[1] Agence Francaise Secur Sanit Aliments, Unite Hyg & Qual Prod Avicoles & Porcins, F-22440 Ploufragen, France

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 29卷 / 06期

关键词：

D O I：

10.1046/j.1472-765X.1999.00658.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Multiplex PCR assay (m-PCR) with three sets of primers was developed for simultaneous identification of Campylobacter jejuni and C. coli. Poultry faecal samples were enriched in Preston broth for 24 h and streaking on selective media was performed before and after enrichment. m-PCR was applied on bacterial cultures harvested from media plates. The data showed a selective effect of Preston broth which favoured the growth of C. coli. Identification of the species by the hippurate hydrolysis test and by the m-PCR was performed on 294 isolates of Campylobacter. The efficiency of the identification by the biochemical test is only 34% in comparison to 100% efficiency with the PCR. The use of our m-PCR in combination with the culture method allowed reliable detection and identification of C. jejuni and C. coli within 3-4 d.

引用

页码：406 / 410

页数：5

共 19 条

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