Investigating the interaction mechanism between zinc and Saccharomyces cerevisiae using combined SEM-EDX and XAFS

被引:43
作者
Chen Can [1 ]
Wang Jianlong [1 ]
机构
[1] Tsinghua Univ, INET, Lab Environm Technol, Beijing 100084, Peoples R China
基金
中国国家自然科学基金;
关键词
Zn2+; Saccharomyces cerevisiae; interaction; XAFS; mechanism;
D O I
10.1007/s00253-008-1415-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The interaction mechanism between zinc and the intact yeast cells of Saccharomyces cerevisiae was investigated by using the scanning electron microscopy with energy-dispersive X-ray analysis, as well as X-ray absorption fine structure spectroscopy (XAFS). Displacement of H+, K+, Mg2+, and Na+ during zinc uptake confirmed the existence of both covalent interactions and ionic interactions between Zn2+ and the microbe. Ion exchange mechanism played a role in zinc uptake. The local environment of Zn accumulated in the intact yeast cells was determined by XAFS, which suggests that the nearest neighboring atom of the bound zinc ion on the biomass is oxygen atom. The adsorbed zinc ion on the intact cells of S. cerevisiae is a tetrahedron structure, with the Zn-O bond length of 1.97 angstrom, and the coordination number is only 3.2 of Zn-O structure in the first shell.
引用
收藏
页码:293 / 299
页数:7
相关论文
共 29 条
[1]   Metal ion coordination at the water-manganite (γ-MnOOH) interface II.: An EXAFS study of zinc(II) [J].
Bochatay, L ;
Persson, P .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2000, 229 (02) :593-599
[2]   Adsorption of cadmium to Bacillus subtilis bacterial cell walls:: A pH-dependent X-ray absorption fine structure spectroscopy study [J].
Boyanov, MI ;
Kelly, SD ;
Kemner, KM ;
Bunker, BA ;
Fein, JB ;
Fowle, DA .
GEOCHIMICA ET COSMOCHIMICA ACTA, 2003, 67 (18) :3299-3311
[3]   BINDING OF HARD AND SOFT METAL-IONS TO RHIZOPUS-ARRHIZUS BIOMASS [J].
BRADY, JM ;
TOBIN, JM .
ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (09) :791-796
[4]   A chemical equilibrium model for metal adsorption onto bacterial surfaces [J].
Fein, JB ;
Daughney, CJ ;
Yee, N ;
Davis, TA .
GEOCHIMICA ET COSMOCHIMICA ACTA, 1997, 61 (16) :3319-3328
[5]   X-ray absorption spectroscopy (XAS) of toxic metal mineral transformations by fungi [J].
Fomina, Marina ;
Charnock, John ;
Bowen, Andrew D. ;
Gadd, Geoffrey M. .
ENVIRONMENTAL MICROBIOLOGY, 2007, 9 (02) :308-321
[6]   Zinc phosphate transformations by the Paxillus involutus/pine ectomycorrhizal association [J].
Fomina, Marina ;
Charnock, John M. ;
Hillier, Stephen ;
Alexander, Ian J. ;
Gadd, Geoffrey M. .
MICROBIAL ECOLOGY, 2006, 52 (02) :322-333
[7]   Cyano-metal complexes uptake by Aspergillus niger [J].
Gomes, NCM ;
Figueira, MM ;
Camargos, ERS ;
Mendonça-Hagler, LCS ;
Dias, JCT ;
Linardi, VR .
BIOTECHNOLOGY LETTERS, 1999, 21 (06) :487-490
[8]   Zinc sorption to three gram-negative bacteria:: Combined titration, modeling, and EXAFS study [J].
Guiné, V ;
Spadini, L ;
Sarret, G ;
Muris, M ;
Delolme, C ;
Gaudet, JP ;
Martins, JMF .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2006, 40 (06) :1806-1813
[9]  
HAN RP, 2004, J ZHENGZHOU U, V36, P86
[10]   Lanthanum biosorption by a Pseudomonas sp.:: equilibrium studies and chemical characterization [J].
Kazy, Sufia K. ;
Das, Susanta K. ;
Sar, Pinaki .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2006, 33 (09) :773-783