Investigating the interaction mechanism between zinc and Saccharomyces cerevisiae using combined SEM-EDX and XAFS

被引：43

作者：

Chen Can ^{[1
]}

Wang Jianlong ^{[1
]}

机构：

[1] Tsinghua Univ, INET, Lab Environm Technol, Beijing 100084, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2008年 / 79卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Zn2+; Saccharomyces cerevisiae; interaction; XAFS; mechanism;

D O I：

10.1007/s00253-008-1415-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The interaction mechanism between zinc and the intact yeast cells of Saccharomyces cerevisiae was investigated by using the scanning electron microscopy with energy-dispersive X-ray analysis, as well as X-ray absorption fine structure spectroscopy (XAFS). Displacement of H+, K+, Mg2+, and Na+ during zinc uptake confirmed the existence of both covalent interactions and ionic interactions between Zn2+ and the microbe. Ion exchange mechanism played a role in zinc uptake. The local environment of Zn accumulated in the intact yeast cells was determined by XAFS, which suggests that the nearest neighboring atom of the bound zinc ion on the biomass is oxygen atom. The adsorbed zinc ion on the intact cells of S. cerevisiae is a tetrahedron structure, with the Zn-O bond length of 1.97 angstrom, and the coordination number is only 3.2 of Zn-O structure in the first shell.

引用

页码：293 / 299

页数：7

共 29 条

[11] An X-ray absorption spectroscopic investigation of the nature of the zinc complex accumulated in Datura innoxia plant tissue culture [J].

Kelly, RA ;

Andrews, JC ;

DeWitt, JG .

MICROCHEMICAL JOURNAL, 2002, 71 (2-3) :231-245

[12] XAFS determination of the bacterial cell wall functional groups responsible for complexation of Cd and U as a function of pH [J].

Kelly, SD ;

Boyanov, MI ;

Bunker, BA ;

Fein, JB ;

Fowle, DA ;

Yee, N ;

Kemner, KM .

JOURNAL OF SYNCHROTRON RADIATION, 2001, 8 (02) :946-948

[13]

Marques PASS, 2000, BIOPROCESS ENG, V23, P135

[14] Experimental measurement of proton, Cd, Pb, Sr, and Zn adsorption onto the fungal species Saccharomyces cerevisiae [J].

Naeem, A. ;

Woertz, J. R. ;

Fein, J. B. .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2006, 40 (18) :5724-5729

[15] Biosorption of zinc from aqueous solutions using biosolids [J].

Norton, L ;

Baskaran, K ;

McKenzie, T .

ADVANCES IN ENVIRONMENTAL RESEARCH, 2004, 8 (3-4) :629-635

[16] EXAFS studies on adsorption-desorption reversibility at manganese oxides-water interfaces I.: Irreversible adsorption of zinc onto manganite (γ-MnOOH) [J].

Pan, G ;

Qin, YW ;

Li, XL ;

Hu, TD ;

Wu, ZY ;

Xie, YN .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2004, 271 (01) :28-34

[17] Environmental and biological applications of extended X-ray absorption fine structure (EXAFS) and X-ray absorption near edge structure (XANES) spectroscopies [J].

Parsons, JG ;

Aldrich, MV ;

Gardea-Torresdey, JL .

APPLIED SPECTROSCOPY REVIEWS, 2002, 37 (02) :187-222

[18] Speciation of Zn associated with diatoms using X-ray absorption spectroscopy [J].

Pokrovsky, OS ;

Pokrovski, GS ;

Gélabert, A ;

Schott, J ;

Boudou, A .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2005, 39 (12) :4490-4498

[19] Adsorption of several metal ions onto a low-cost biosorbent: Kinetic and equilibrium studies [J].

Reddad, Z ;

Gerente, C ;

Andres, Y ;

Le Cloirec, P .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2002, 36 (09) :2067-2073

[20] Structural determination of Zn and Pb binding sites in Penicillium chrysogenum cell walls by EXAFS spectroscopy [J].

Sarret, G ;

Manceau, A ;

Spadini, L ;

Roux, JC ;

Hazemann, JL ;

Soldo, Y ;

Eybert-Berard, L ;

Menthonnex, JJ .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1998, 32 (11) :1648-1655

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