Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone

被引：12

作者：

De Stasio, G

Gilbert, B

Perfetti, L

Hansen, R

Mercanti, D

Ciotti, MT

Andres, R

White, VE

Perfetti, P

Margaritondo, G

机构：

[1] CNR, Ist Struttura Mat, I-00133 Rome, Italy

[2] Ecole Polytech Fed Lausanne, Inst Phys Appl, PH Ecublens, CH-1015 Lausanne, Switzerland

[3] Univ Roma Tor Vergata, Rome, Italy

[4] Univ Wisconsin, Ctr Synchrotron Radiat, Stoughton, WI 53589 USA

[5] CNR, Ist Neurobiol, I-00137 Rome, Italy

[6] Paul Scherrer Inst, CH-5232 Villigen, Switzerland

[7] CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA

[8] Sincrotrone Trieste SCpA, Trieste, Italy

来源：

ANALYTICAL BIOCHEMISTRY | 1999年 / 266卷 / 02期

关键词：

synchrotron spectromicroscopy; microchemical analysis; MEPHISTO; ashing; incineration; trace element;

D O I：

10.1006/abio.1998.2901

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied a new approach to cell ashing based on illuminating the specimens with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurobiological specimens. Our results demonstrate that carbon is removed, whereas phosphorus, calcium, potassium, and sulfur are retained and their relative concentrations are enhanced. Applied to trace elements, this technique will enhance their practical detectability. (C) 1999 Academic Press.

引用

页码：174 / 180

页数：7

共 17 条

[1] ALOISI F, 1985, J NEUROSCI, V5, P2001
[2] [Anonymous], 1979, HDB XRAY PHOTOELECTR
[3] BELLANGER JR, 1995, J AOAC INT, V78, P477
[4] EB-1089, A CALCITRIOL ANALOG, DECREASES FETAL CALCIUM CONTENT WHEN INJECTED INTO PREGNANT RATS
DAVICCO, MJ
COXAM, V
GAUMET, N
LEBECQUE, P
BARLET, JP
[J]. EXPERIMENTAL PHYSIOLOGY, 1995, 80 (03) : 449 - 456
[5] DE STASIO G, 1993, NEUROREPORT, V4, P1175
[6] The effect of ashing on cells: Spectromicroscopy of physiological elements
De Stasio, G
Capozi, M
Droubay, TC
Mercanti, D
Ciotti, MT
Lorusso, GF
Andres, R
Suda, T
Perfetti, P
Tonner, BP
Margaritondo, G
[J]. ANALYTICAL BIOCHEMISTRY, 1997, 252 (01) : 106 - 109
[7] MEPHISTO: Performance tests of a novel synchrotron imaging photoelectron spectromicroscope
De Stasio, G
Capozi, M
Lorusso, GF
Baudat, PA
Droubay, TC
Perfetti, P
Margaritondo, G
Tonner, BP
[J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 1998, 69 (05) : 2062 - 2066
[8] PHOTOEMISSION SPECTROMICROSCOPY OF NEURONS
DE STASIO, G
HARDCASTLE, S
KORANDA, SF
TONNER, BP
MERCANTI, D
CIOTTI, MT
PERFETTI, P
MARGARITONDO, G
[J]. PHYSICAL REVIEW E, 1993, 47 (03) : 2117 - 2121
[9] CLOSE SIMILARITY BETWEEN PHOTOELECTRIC YIELD AND PHOTOABSORPTION SPECTRA IN SOFT-X-RAY RANGE
GUDAT, W
KUNZ, C
[J]. PHYSICAL REVIEW LETTERS, 1972, 29 (03) : 169 - &
[10] CLEANING OF OPTICAL-SURFACES WITH PHOTOGENERATED REACTANTS
HANSEN, RWC
WOLSKE, J
WALLACE, D
BISSEN, M
[J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 1994, 347 (1-3) : 249 - 253

← 1 2 →