Detection of antibodies against viral capsid proteins of human herpesvirus 8 in AIDS-associated Kaposi's sarcoma

被引:30
作者
Andre, S
Schatz, O
Bogner, JR
Zeichhardt, H
StofflerMeilicke, M
Jahn, HU
Ullrich, R
Sonntag, AK
Kehm, R
Haas, J
机构
[1] UNIV MUNICH,MAX VON PETTENKOFER INST VIROL,GENZENTRUM,D-81377 MUNICH,GERMANY
[2] UNIV MUNICH,MED POLIKLIN,D-80336 MUNICH,GERMANY
[3] FREE UNIV BERLIN,KLINIKUM BENJAMIN FRANKLIN,INST KLIN & EXPT VIROL,D-12203 BERLIN,GERMANY
[4] FREE UNIV BERLIN,KLINIKUM BENJAMIN FRANKLIN,MED KLIN,D-12200 BERLIN,GERMANY
[5] UNIV HEIDELBERG,HAUTKLIN,D-69115 HEIDELBERG,GERMANY
[6] UNIV HEIDELBERG,INST MED VIROL,D-69120 HEIDELBERG,GERMANY
来源
JOURNAL OF MOLECULAR MEDICINE-JMM | 1997年 / 75卷 / 02期
关键词
antibody; capsid antigens; HHV-8; Kaposi's sarcoma;
D O I
10.1007/s001090050099
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Sequences of a new herpesvirus with homology to gammaherpesvirinae were recently identified in AIDS-associated Kaposi's sarcoma (KS). Subsequently this novel virus, called KS-associated virus (KSHV) or human herpesvirus (HHV) 8 was detected in classical KS and AIDS-associated body cavity based lymphomas by polymerase chain reaction, In this report major and minor capsid proteins of HHV-8 were molecularly cloned and produced as recombinant proteins in Escherichia coli. Sera from 69 HIV-1 infected patients with KS, 30 HIV-1 infected patients without KS and 106 control individuals were tested by enzyme-linked immunosorbent assay for anti-HHV-8 capsid IgM and IgG antibodies. Sera from four patients were tested over periods ranging from 18 months to 6 years. IgG antibodies directed against HHV-8 capsid antigens were detected in patients with AIDS-associated KS and in some AIDS patients without KS. Seroconversion with IgM and IgG antibodies directed against HHV-8 capsid proteins occurred more than 1 year prior to diagnosis of KS. In a considerable portion of KS patients no IgM or IgG antibodies against HHV-8 capsid proteins were detected. In these patients there was an inverse relationship between antibodies against HHV-8orf26 and the CD4/CD8 ratio, suggesting that the inconsistency of anti-HHV-8orf26 antibodies is due at least partly to an impaired immune response, No reactivity against HHV-8 capsid antigens was detected in the vast majority of sera from HIV-negative control individuals. Our findings indicate that a specific humoral immune response against capsid proteins is raised in HHV-8 infected individuals, and that anti-capsid antibodies can be used to diagnose HHV-8 infection. The correlation between occurrence of anti-HHV-8 antibodies and KS supports the hypothesis of a causative role of HHV-8.
引用
收藏
页码:145 / 152
页数:8
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