Heterologous Expression of 3-O-Deacylase in Acinetobacter baumannii Modulates the Endotoxicity of Lipopolysaccharide

被引：5

作者：

Badmasti, Farzad ^{[1
]}

Shahcheraghi, Fereshteh ^{[1
]}

Siadat, Seyed Davar ^{[1
]}

Bouzari, Saeid ^{[2
]}

Ajdary, Soheila ^{[3
]}

Amin, Mohsen ^{[5
]}

Halabian, Raheleh ^{[4
]}

Fooladi, Abbas Ali Imani ^{[4
]}

机构：

[1] Baqiyatallah Univ Med Sci, Dept Bacteriol, Tehran, Iran

[2] Baqiyatallah Univ Med Sci, Dept Mol Biol, Tehran, Iran

[3] Baqiyatallah Univ Med Sci, Pasteur Inst Iran, Dept Immunol, Tehran, Iran

[4] Baqiyatallah Univ Med Sci, Appl Microbiol Res Ctr, Tehran, Iran

[5] Univ Tehran Med Sci, Fac Pharm, Dept Drug & Food Control, Tehran, Iran

来源：

JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY | 2015年 / 25卷 / 01期

关键词：

Acinetobacter baumannii ATCC 19606; 3-O-deacylase enzyme; Lipopolysaccharide; Lipid A; Endotoxicity; Outer membrane vesicle; Inactivated whole cell; TOLL-LIKE RECEPTOR-4; PSEUDOMONAS-AERUGINOSA; MODIFYING ENZYMES; PAGL; LPS; PERTUSSIS; VACCINE;

D O I：

10.1159/000371815

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The lipopolysaccharide (LPS) of Acinetobacter baumannii is a potent stimulator of proinflammatory cytokines, such as interleukin-6 (IL-6). The 3-O-deacylase (PagL)-modifying enzyme that removes the 3-O-linked acyl chain from the disaccharide backbone of lipid A provides the opportunity to develop a new therapeutic compound that could reduce detrimental inflammatory responses. The plasmid pMMB66EH-PagL obtained by recombinant DNA technology was electroporated into A. baumannii ATCC 19606. Compared with wild-type LPS, outer membrane vesicles and inactivated whole cells of engineered bacteria had a statistically significant decreased ability to produce IL-6. Structural analysis of lipid A by negative-ion matrix-assisted laser desorption/ionization time-of-flight mass spectrometry revealed that the profile of lipid A fractions under PagL expression was changed. Taken together, our data showed that recombinant penta-acylated lipid A had less immunoreactivity and that the tetra-acylated version of lipid A with TLR4 antagonist activity decreased the induction of IL-6 production in the murine macrophage cell line J774 A.1. (C) 2015 S. Karger AG, Basel

引用

页码：37 / 44

页数：8

共 24 条

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