Surface enhanced Raman spectroscopic detection of cancer biomarkers in intact cellular specimens

被引：13

作者：

Ansari, DO ^{[1
]}

Stuart, DA ^{[1
]}

Nie, SM ^{[1
]}

机构：

[1] Georgia Inst Technol, Dept Biomed Engn, Atlanta, GA 30322 USA

来源：

Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III | 2005年 / 5699卷

关键词：

surface enhanced Raman scattering; SERS; optical encoding; immunoassay; cancer; diagnosis; biomarkers; nanoparticles; nanotechnology;

D O I：

10.1117/12.591178

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have developed a system for the direct spectroscopic identification of protein biomarkers in biological samples. By conjugating Raman dyes and biomolecular targeting agents to gold nanoparticles, we have produced highly selective optically encoded probes. The nanostructures are grown within a cellular sample to generate a surface that is highly active for surface enhanced Raman spectroscopy (SERS). Both in vitro characterizations of SERS detection as well as the sensitive and specific detection of cancer biomarkers in cultured cancer cells have been demonstrated.

引用

页码：82 / 90

页数：9

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