Amplified electrocatalysis at DNA-modified nanowires

被引：97

作者：

Lapierre-Devlin, MA ^{[1
]}

Asher, CL ^{[1
]}

Taft, BJ ^{[1
]}

Gasparac, R ^{[1
]}

Roberts, MA ^{[1
]}

Kelley, SO ^{[1
]}

机构：

[1] Boston Coll, Eugene F Merkert Chem Ctr, Chestnut Hill, MA 02467 USA

来源：

NANO LETTERS | 2005年 / 5卷 / 06期

关键词：

D O I：

10.1021/nl050483a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Arrayed gold nanowires are a novel and useful platform for electrochemical DNA detection. Pilot studies testing the use of these templated structures with an electrocatalytic reporter system revealed that very low detection thresholds for target DNA sequences can be obtained. One factor contributing to the heightened sensitivity is the high signal-to-noise ratio achieved with the large electrocatalytic signals observed at DNA-modified nanowires. Here, we explain the improved sensitivity with evidence illustrating that electrocatalysis at DNA-modified nanostructures generates amplified signals that are significantly larger than those observed at bulk gold surfaces. The results presented strongly suggest that the three-dimensional architectures of the nanowires facilitate the electrocatalytic reaction because of enhanced diffusion occurring around these structures. Effects unique to the nanoscale are shown to underlie the utility of nanowires for DNA biosensing.

引用

页码：1051 / 1055

页数：5

共 15 条

[1] KINETIC-BEHAVIOR TO BE EXPECTED FROM OUTER-SPHERE REDOX CATALYSTS CONFINED WITHIN POLYMERIC FILMS ON ELECTRODE SURFACES [J].

ANSON, FC .

JOURNAL OF PHYSICAL CHEMISTRY, 1980, 84 (25) :3336-3338

[2] DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS [J].

BEAUCAGE, SL ;

CARUTHERS, MH .

TETRAHEDRON LETTERS, 1981, 22 (20) :1859-1862

[3] Mutation detection by electrocatalysis at DNA-modified electrodes [J].

Boon, EM ;

Ceres, DM ;

Drummond, TG ;

Hill, MG ;

Barton, JK .

NATURE BIOTECHNOLOGY, 2000, 18 (10) :1096-1100

[4]

Chapuzet JM, 1998, FRONT ELECT, P155

[5] RESPONSE OF MICROVOLTAMMETRIC ELECTRODES TO HOMOGENEOUS CATALYTIC AND SLOW HETEROGENEOUS CHARGE-TRANSFER REACTIONS [J].

DAYTON, MA ;

EWING, AG ;

WIGHTMAN, RM .

ANALYTICAL CHEMISTRY, 1980, 52 (14) :2392-2396

[6] Rapid amperometric verification of PCR amplification of DNA [J].

de Lumley-Woodyear, T ;

Campbell, CN ;

Freeman, E ;

Freeman, A ;

Georgiou, G ;

Heller, A .

ANALYTICAL CHEMISTRY, 1999, 71 (03) :535-538

[7]

F Allen J Bard L.R., 2001, Electrochemical Methods: Fundamentals and Applications

[8] Ultrasensitive electrocatalytic DNA detection at two- and three-dimensional nanoelectrodes [J].

Gasparac, R ;

Taft, BJ ;

Lapierre-Devlin, MA ;

Lazareck, AD ;

Xu, JM ;

Kelley, SO .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (39) :12270-12271

[9]

Jarvi TD, 1998, FRONT ELECT, P75

[10] Single-base mismatch detection based on charge transduction through DNA [J].

Kelley, SO ;

Boon, EM ;

Barton, JK ;

Jackson, NM ;

Hill, MG .

NUCLEIC ACIDS RESEARCH, 1999, 27 (24) :4830-4837

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