Ultrasensitive electrocatalytic DNA detection at two- and three-dimensional nanoelectrodes

被引：168

作者：

Gasparac, R

Taft, BJ

Lapierre-Devlin, MA

Lazareck, AD

Xu, JM

Kelley, SO ^{[1
]}

机构：

[1] Boston Coll, Eugene F Merkert Chem Ctr, Chestnut Hill, MA 02467 USA

[2] Brown Univ, Div Engn, Providence, RI 02912 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 39期

关键词：

D O I：

10.1021/ja0458221

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Electrochemical DNA detection systems are an attractive approach to the development of multiplexed, high-throughput DNA analysis systems for clinical and research applications. We have engineered a new class of nanoelectrode ensembles (NEEs) that constitute a useful platform for biomolecular electrochemical sensing. High-sensitivity DNA detection was achieved at oligonucleotide-functionalized NEEs using a label-free electrocatalytic assay. Attomole levels of DNA were detected using the NEEs, validating the promise of nanoarchitectures for ultrasensitive biosensing. Copyright © 2004 American Chemical Society.

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页码：12270 / 12271

页数：2

相关论文

共 18 条

[1] HELICOBACTER-PYLORI AND THE PATHOGENESIS OF GASTRODUODENAL INFLAMMATION [J].

BLASER, MJ .

JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (04) :626-633

[2] Electrochemistry of phenothiazine and methylviologen biosensor electron-transfer mediators at nanoelectrode ensembles [J].

Brunetti, B ;

Ugo, P ;

Moretto, LM ;

Martin, CR .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2000, 491 (1-2) :166-174

[3] Electrochemical detection of DNA triplet repeat expansion [J].

Fojta, M ;

Havran, L ;

Vojtiskova, M ;

Palecek, E .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (21) :6532-6533

[4] Electrochemical preparation and surface properties of gold nanowire arrays formed by the template technique [J].

Forrer, P ;

Schlottig, F ;

Siegenthaler, H ;

Textor, M .

JOURNAL OF APPLIED ELECTROCHEMISTRY, 2000, 30 (05) :533-541

[5] Protein electrochemistry using aligned carbon nanotube arrays [J].

Gooding, JJ ;

Wibowo, R ;

Liu, JQ ;

Yang, WR ;

Losic, D ;

Orbons, S ;

Mearns, FJ ;

Shapter, JG ;

Hibbert, DB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (30) :9006-9007

[6] Detection of attomole quantitites of DNA targets on gold microelectrodes by electrocatalytic nucleobase oxidation [J].

Gore, MR ;

Szalai, VA ;

Ropp, PA ;

Yang, IV ;

Silverman, JS ;

Thorp, HH .

ANALYTICAL CHEMISTRY, 2003, 75 (23) :6586-6592

[7] Electrocatalytic detection of pathogenic DNA sequences and antibiotic resistance markers [J].

Lapierre, MA ;

O'Keefe, M ;

Taft, BJ ;

Kelley, SO .

ANALYTICAL CHEMISTRY, 2003, 75 (22) :6327-6333

[8] Highly-ordered carbon nanotube arrays for electronics applications [J].

Li, J ;

Papadopoulos, C ;

Xu, JM ;

Moskovits, M .

APPLIED PHYSICS LETTERS, 1999, 75 (03) :367-369

[9] FABRICATION AND EVALUATION OF NANOELECTRODE ENSEMBLES [J].

MENON, VP ;

MARTIN, CR .

ANALYTICAL CHEMISTRY, 1995, 67 (13) :1920-1928

[10] Voltammetry of redox analytes at trace concentrations with nanoelectrode ensembles [J].

Moretto, LM ;

Pepe, N ;

Ugo, P .

TALANTA, 2004, 62 (05) :1055-1060